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001-es BibID:	BIBFORM078886
Első szerző:	Matta Csaba (molekuláris biológus, genetikus, angol szakfordító)
Cím:	Osteogenic differentiation of human bone marrow-derived mesenchymal stem cells is enhanced by an aragonite scaffold / Csaba Matta, Csilla Szűcs-Somogyi, Elizaveta Kon, Dror Robinson, Tova Neufeld, Nir Altschuler, Agnes Berta, László Hangody, Zoltán Veréb, Róza Zákány
Dátum:	2019
ISSN:	0301-4681
Megjegyzések:	Bone graft substitutes and bone void fillers are predominantly used to treat bone defects and bone fusion in orthopaedic surgery. Some aragonite-based scaffolds of coralline exoskeleton origin exhibit osteoconductive properties and are described as useful bone repair scaffolds. The purpose of this study was to evaluate the in vitro osteogenic potential of the bone phase of a novel aragonite-based bi-phasic osteochondral scaffold (Agili-C?, CartiHeal Ltd.) using adult human bone marrow-derived mesenchymal stem cells (MSCs). Analyses were performed at several time intervals: 3, 7, 14, 21, 28 and 42 days post-seeding. Osteogenic differentiation was assessed by morphological characterisation using light microscopy after Alizarin red and von Kossa staining, and scanning electron microscopy. The transcript levels of alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), bone gamma-carboxyglutamate (BGLAP), osteonectin (SPARC) and osteopontin (SPP1) were determined by quantitative PCR. Proliferation was assessed by a thymidine incorporation assay and proliferating cell nuclear antigen (PCNA) immunocytochemistry. Our results demonstrate that the bone phase of the bi-phasic aragonite-based scaffold supports osteogenic differentiation and enhanced proliferation of bone marrow-derived MSCs at both the molecular and histological levels. The scaffold was colonized by differentiating MSCs, suggesting its suitability for incorporation into bone voids to accelerate bone healing, remodelling and regeneration. The mechanism of osteogenic differentiation involves scaffold surface modification with de novo production of calcium phosphate deposits, as revealed by energy dispersive spectroscopy (EDS) analyses. This novel coral-based scaffold may promote the rapid formation of high quality bone during the repair of osteochondral lesions.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Differentiation. - 107 (2019), p. 24-34. -
További szerzők:	Somogyi Csilla (1983-) (biológus, angol-magyar szakfordító) Kon, Elizaveta Robinson, Dror Neufeld, Tova Altschuler, Nir Berta Ágnes Ida Hangody László Veréb Zoltán Zákány Róza (1963-) (anatómus-, kötőszövetbiológus)
Pályázati támogatás:	Bolyai János ösztöndíj Egyéb MTA Prémium ösztöndíj Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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