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001-es BibID:BIBFORM079298
035-os BibID:(PMID)31158411 (WoS)000473008800003 (Scopus)85067187461
Első szerző:Erdős Edina (klinikai laboratóriumi kutató)
Cím:COUP-TFII is a modulator of cell-type-specific genetic programs based on genomic localization maps / Edina Erdős, Bálint László Bálint
Dátum:2019
ISSN:0168-1656
Megjegyzések:Chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) is a member of the steroid/thyroid hormone receptor superfamily, but its ligand has not yet been identified. Little is known about the role of the COUP-TFII nuclear receptor in cancer cells. In this study, we mapped the cistrome of COUP-TFII in three different cancer cells, namely breast cancer cells (MCF-7), myelogenous leukaemia cells (K562) and liver cancer cells (HepG2) using publicly available ChIP-seq data. Our results show that COUP-TFII co-localises with master transcription factors (TFs) in a cell-specific manner such as estrogen receptor alpha in MCF-7, hepatocyte nuclear factor alpha in HepG2, and GATA-binding factor in K562, while the shared, non-specific COUP-TFII binding sites are co-occupied by CTCF. We identified chromatin environments for these COUP-TFII and master TF co-bound sites together with COUP-TFII and CTCF co-bound sites. Our results show that COUP-TFII and master TF co-bound sites are marked with active enhancer specific histone modifications (H3K27ac and H3K4me1), while COUP-TFII and CTCF co-bound sites reveal active promoter specific histone marks (H3K27ac and H3K4me3). These results describe the genomic context and role of COUP-TFII in the cell-type specific transcriptional programs. Furthermore, we report that the VEGFA gene regulated by shared COUP-TFII and CTCF co-bound regulatory elements is involved in long-range looping in a cell-type-independent manner. These findings provide a genomic insight into the regulation and angiogenic role of COUP-TFII.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
COUP-TFII/NR2F2
Cell-type-specific regulation
Estrogen receptor alpha (ERα)
Hepatocyte nuclear factor 4 alpha (HNF4α)
Master transcription factor
VEGFA
Megjelenés:Journal Of Biotechnology. - 301 (2019), p. 11-17. -
További szerzők:Bálint Bálint László (1971-) (kutató orvos)
Pályázati támogatás:EFOP-3.6.1-16-2016-00022
EFOP
GINOP-2.3.3-15-2016-00007
GINOP
NKFIH K-129166
OTKA
TÁMOP 4.2.4.A/2-11-1-2012-0001
TÁMOP
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DOI
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