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001-es BibID:	BIBFORM080103
035-os BibID:	(cikkazonosító)3361 (scopus)85071306288 (wos)000477041100245
Első szerző:	Pierantozzi, Enrico
Cím:	Calcium Homeostasis Is Modified in Skeletal Muscle Fibers of Small Ankyrin1 Knockout Mice / Enrico Pierantozzi, Péter Szentesi, Dána Al-Gaadi, Tamás Oláh, Beatrix Dienes, Mónika Sztretye, Daniela Rossi, Vincenzo Sorrentino, László Csernoch
Dátum:	2019
ISSN:	1661-6596 1422-0067
Megjegyzések:	Small Ankyrins (sAnk1) are muscle-specific isoforms generated by the Ank1 gene that participate in the organization of the sarcoplasmic reticulum (SR) of striated muscles. Accordingly, the volume of SR tubules localized around the myofibrils is strongly reduced in skeletal muscle fibers of 4- and 10-month-old sAnk1 knockout (KO) mice, while additional structural alterations only develop with aging. To verify whether the lack of sAnk1 also alters intracellular Ca2+ handling, cytosolic Ca2+ levels were analyzed in stimulated skeletal muscle fibers from 4- and 10-month-old sAnk1 KO mice. The SR Ca2+ content was reduced in sAnk1 KO mice regardless of age. The amplitude of the Ca2+ transients induced by depolarizing pulses was decreased in myofibers of sAnk1 KO with respect to wild type (WT) fibers, while their voltage dependence was not affected. Furthermore, analysis of spontaneous Ca2+ release events (sparks) on saponin-permeabilized muscle fibers indicated that the frequency of sparks was significantly lower in fibers from 4-month-old KO mice compared to WT. Furthermore, both the amplitude and spatial spread of sparks were significantly smaller in muscle fibers from both 4- and 10-month-old KO mice compared to WT. These data suggest that the absence of sAnk1 results in an impairment of SR Ca2+ release, likely as a consequence of a decreased Ca2+ store due to the reduction of the SR volume in sAnk1 KO muscle fibers.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban sarcoplasmic reticulum sAnk1 calcium release spark
Megjelenés:	International Journal of Molecular Sciences. - 20 : 13 (2019), p. 3361. -
További szerzők:	Szentesi Péter (1967-) (élettanász) Al-Gaadi, Dana (1991-) Oláh Tamás (1983-) (élettanász) Dienes Beatrix (1972-) (élettanász, molekuláris biológus) Sztretye Mónika (1981-) (élettanász, elektrofiziológus) Rossi, Daniela Sorrentino, Vincenzo Csernoch László (1961-) (élettanász)
Pályázati támogatás:	NKFIH K-115461 NKFIH GINOP-2.3.2-15-2016-00040 GINOP EFOP-3.6.2-16-2017-00006 EFOP ÚNKP-18-4-DE-157 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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