Találati lista | Tudóstér

001-es BibID:	BIBFORM080605
035-os BibID:	(cikkazonosító)E4119 (PMID)31450846 (scopus)85071765729 (wos)000486888400051
Első szerző:	Dvorská, Dana
Cím:	Aberrant Methylation Status of Tumour Suppressor Genes in Ovarian Cancer Tissue and Paired Plasma Samples / Dana Dvorská, Dušan Braný, Bálint Nagy, Marián Grendár , Robert Poka, Beáta Soltész, Marianna Jagelková, Katarína Zelinová, Zora Lasabová, Pavol Zubor, Zuzana Danková
Dátum:	2019
ISSN:	1661-6596 1422-0067
Megjegyzések:	Ovarian cancer is a highly heterogeneous disease and its formation is affected by many epidemiological factors. It has typical lack of early signs and symptoms, and almost 70% of ovarian cancers are diagnosed in advanced stages. Robust, early and non-invasive ovarian cancer diagnosis will certainly be beneficial. Herein we analysed the regulatory sequence methylation profiles of the RASSF1, PTEN, CDH1 and PAX1 tumour suppressor genes by pyrosequencing in healthy, benign and malignant ovarian tissues, and corresponding plasma samples. We recorded statistically significant higher methylation levels (p < 0.05) in the CDH1 and PAX1 genes in malignant tissues than in controls (39.06 ? 18.78 versus 24.22 ? 6.93; 13.55 ? 10.65 versus 5.73 ? 2.19). Higher values in the CDH1 gene were also found in plasma samples (22.25 ? 14.13 versus 46.42 ? 20.91). A similar methylation pattern with positive correlation between plasma and benign lesions was noted in the CDH1 gene (r = 0.886, p = 0.019) and malignant lesions in the PAX1 gene (r = 0.771, p < 0.001). The random forest algorithm combining methylation indices of all four genes and age determined 0.932 AUC (area under the receiver operating characteristic (ROC) curve) prediction power in the model classifying malignant lesions and controls. Our study results indicate the effects of methylation changes in ovarian cancer development and suggest that the CDH1 gene is a potential candidate for non-invasive diagnosis of ovarian cancer.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk liquid biopsy pirosequencing ovarian cancer
Megjelenés:	International Journal of Molecular Sciences. - 20 : 17 (2019), p. 1-19. -
További szerzők:	Braný, Dušan Nagy Bálint (1956-) (molekuláris genetikus) Grendar, Marian Póka Róbert (1960-) (szülész-nőgyógyász, klinikai onkológus) Soltész Beáta (1987-) (molekuláris biológus) Jagelková, Marianna Zelinová, Katarína Lasabova, Zora Zubor, Pavol Danková, Zuzana
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: