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001-es BibID:	BIBFORM080933
035-os BibID:	(PMID) 31227739 (cikkazonosító)9018 (WOS)000472477300017 (Scopus)85067811165
Első szerző:	Matta Csaba (molekuláris biológus, genetikus, angol szakfordító)
Cím:	Molecular phenotyping of the surfaceome of migratory chondroprogenitors and mesenchymal stem cells using biotinylation, glycocapture and quantitative LC-MS/MS proteomic analysis / Csaba Matta, David J. Boocock, Christopher R. Fellows, Nicolai Miosge, James E. Dixon, Susan Liddell, Julia Smith, Ali Mobasheri
Dátum:	2019
ISSN:	2045-2322
Megjegyzések:	The complement of cell surface proteins, collectively referred to as the surfaceome, is a useful indicator of normal differentiation processes, and the development of pathologies such as osteoarthritis (OA). We employed biochemical and proteomic tools to explore the surfaceome and to define biomarkers in chondrogenic progenitor cells (CPC) derived from human OA knee articular cartilage. These cells have great therapeutic potential, but their unexplored biology limits their clinical application. We performed biotinylation combined with glycocapture and high throughput shotgun proteomics to define the surface proteome of human bone marrow mesenchymal stem cells (MSCs) and human CPCs. We prepared cell surface protein-enriched fractions from MSCs and CPCs, and then a proteomic approach was used to compare and evaluate protein changes between undifferentiated MSCs and CPCs. 1256 proteins were identified in the study, of which 791 (63%) were plasma membrane, cell surface or extracellular matrix proteins. Proteins constituting the surfaceome were annotated and categorized. Our results provide, for the first time, a repository of quantitative proteomic data on the surfaceome of two closely related cell types relevant to cartilage biology and OA. These results may provide novel insights into the transformation of the surfaceome during chondrogenic differentiation and phenotypic changes during OA development.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Scientific Reports. - 9 : 1 (2019), p. 1-15. -
További szerzők:	Boocock, David J. Fellows, Christopher R. Miosge, Nicolai Dixon, James E. Liddell, Susan Smith, Julia R. Mobasheri, Ali
Pályázati támogatás:	Prémium Posztdoktori Ösztöndíj MTA Debreceni Egyetem Bridging Fund Ösztöndíj Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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