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001-es BibID:	BIBFORM085971
035-os BibID:	(cikkazonosító)e0231223 (WOS)000536011400032 (Scopus)85083478527
Első szerző:	Nánási Péter Pál ifj. (sejtbiológus)
Cím:	Doxorubicin induces large-scale and differential H2A and H2B redistribution in live cells / Nánási Péter, Imre László, Firouzi Niaki Erfaneh, Bosire Rosevalentine, Mocsár Gábor, Türk-Mázló Anett, Ausio Juan, Szabó Gábor
Dátum:	2020
ISSN:	1932-6203
Megjegyzések:	We observed prominent effects of doxorubicin (Dox), an anthracycline widely used in anticancer therapy, on the aggregation and intracellular distribution of both partners of the H2AH2B dimer, with marked differences between the two histones. Histone aggregation, assessed by Laser Scanning Cytometry via the retention of the aggregates in isolated nuclei, was observed in the case of H2A. The dominant effect of the anthracycline on H2B was its massive accumulation in the cytoplasm of the Jurkat leukemia cells concomitant with its disappearance from the nuclei, detected by confocal microscopy and mass spectrometry. A similar effect of the anthracycline was observed in primary human lymphoid cells, and also in monocyte-derived dendritic cells that harbor an unusually high amount of H2B in their cytoplasm even in the absence of Dox treatment. The nucleo-cytoplasmic translocation of H2B was not affected by inhibitors of major biochemical pathways or the nuclear export inhibitor leptomycin B, but it was completely diminished by PYR-41, an inhibitor with pleiotropic effects on protein degradation pathways. Dox and PYR-41 acted synergistically according to isobologram analyses of cytotoxicity. These large-scale effects were detected already at Dox concentrations that may be reached in the typical clinical settings, therefore they can contribute both to the anti-cancer mechanism and to the side-effects of this anthracycline.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Plos One. - 15 : 4 (2020), p. 1-23. -
További szerzők:	Imre László (1979-) (biológus) Firouzi Niaki, Erfaneh Bosire, Rosevalentine (1988-) (biotechnológus) Mocsár Gábor (1981-) (biofizikus) Türk-Mázló Anett (1989-) (molekuláris biológus) Ausio, Juan Szabó Gábor (1953-) (biofizikus)
Pályázati támogatás:	GINOP-2.3.3-15-2016-00020 GINOP GINOP-2.3.2-15-2016-00044 GINOP K128770 OTKA COST EuroCellNet CA15214 Egyéb Richter Gedeon Talentum Foundation Egyéb Stipendium Hungaricum, Tempus Public foundation Egyéb
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