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001-es BibID:	BIBFORM091513
035-os BibID:	(cikkazonosító)1648 (scopus)85100462177 (wos)000623909700001
Első szerző:	Borbély Adina
Cím:	Structural Characterization of Daunomycin-Peptide Conjugates by Various Tandem Mass Spectrometric Techniques / Adina Borbély, Lilla Pethő, Ildikó Szabó, Mohammed Al-Majidi, Arnold Steckel, Tibor Nagy, Sándor Kéki, Gergő Kalló, Éva Csősz, Gábor Mező, Gitta Schlosser
Dátum:	2021
ISSN:	1661-6596 1422-0067
Megjegyzések:	The use of peptide-drug conjugates has generated wide interest as targeted antitumor therapeutics. The anthracycline antibiotic, daunomycin, is a widely used anticancer agent and it is often conjugated to different tumor homing peptides. However, comprehensive analytical characterization of these conjugates via tandem mass spectrometry (MS/MS) is challenging due to the lability of the O-glycosidic bond and the appearance of MS/MS fragment ions with little structural information. Therefore, we aimed to investigate the optimal fragmentation conditions that suppress the prevalent dissociation of the anthracycline drug and provide good sequence coverage. In this study, we comprehensively compared the performance of common fragmentation techniques, such as higher energy collisional dissociation (HCD), electron transfer dissociation (ETD), electron-transfer higher energy collisional dissociation (EThcD) and matrix-assisted laser desorption/ionization-tandem time-of-flight (MALDI-TOF/TOF) activation methods for the structural identification of synthetic daunomycin-peptide conjugates by high-resolution tandem mass spectrometry. Our results showed that peptide backbone fragmentation was inhibited by applying electron-based dissociation methods to conjugates, most possibly due to the "electron predator" effect of the daunomycin. We found that efficient HCD fragmentation was largely influenced by several factors, such as amino acid sequences, charge states and HCD energy. High energy HCD and MALDI-TOF/TOF combined with collision induced dissociation (CID) mode are the methods of choice to unambiguously assign the sequence, localize different conjugation sites and differentiate conjugate isomers.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk MALDI anthracyclines bioconjugates collision-induced dissociation electron predator high resolution mass spectrometry
Megjelenés:	International Journal Of Molecular Sciences. - 22 : 4 (2021), p. 1-15. -
További szerzők:	Pethő Lilla Szabó Ildikó Al-Majidi, Mohammed Steckel Arnold Nagy Tibor (1988-) (vegyész) Kéki Sándor (1964-) (polimer kémikus) Kalló Gergő (1989-) (molekuláris biológus) Csősz Éva (1977-) (biokémikus, molekuláris biológus) Mező Gábor (1959-) (vegyész) Schlosser Gitta
Pályázati támogatás:	NVKP_16-1-2016-0036 Egyéb Lendület Momentum Program Egyéb VEKOP- 387 2.3.3-15-2017-00020 Egyéb GINOP-2.3.3-15-2016-00020 GINOP GINOP-2.3.3-15-2016-00021 GINOP
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