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001-es BibID:	BIBFORM091922
035-os BibID:	(cikkazonosító)107692
Első szerző:	Krishnarjuna, Bankala
Cím:	A disulfide-stabilised helical hairpin fold in acrorhagin I : an emerging structural motif in peptide toxins / Bankala Krishnarjuna, Punnepalli Sunanda, Jessica Villegas-Moreno, Agota Csoti, Rodrigo A. V. Morales, Dorothy C. C. Wai, Gyorgy Panyi, Peter Prentis, Raymond S. Norton
Dátum:	2021
ISSN:	1047-8477
Megjegyzések:	Acrorhagin I (U-AITX-Aeq5a) is a disulfide-rich peptide identified in the aggressive organs (acrorhagi) of the sea anemone Actinia equina. Previous studies (Toxicon 2005, 46:768-74) found that the peptide is toxic in crabs, although the structural and functional properties of acrorhagin I have not been reported. In this work, an Escherichia coli (BL21 strain) expression system was established for the preparation of 13C,15N-labelled acrorhagin I, and the solution structure was determined using NMR spectroscopy. Structurally, acrorhagin I is similar to B-IV toxin from the marine worm Cerebratulus lacteus (PDB id 1VIB), with a well-defined helical hairpin structure stabilised by four intramolecular disulfide bonds. The recombinant peptide was tested in patch-clamp electrophysiology assays against voltage-gated potassium and sodium channels, and in bacterial and fungal growth inhibitory assays and haemolytic assays. Acrorhagin I was not active against any of the ion channels tested and showed no activity in functional assays, indicating that this peptide may possess a different biological function. Metal ion interaction studies using NMR spectroscopy showed that acrorhagin I bound zinc and nickel, suggesting that its function might be modulated by metal ions or that it may be involved in regulating metal ion levels and their transport. The similarity between the structure of acrorhagin I and that of B-IV toxin from a marine worm suggests that this fold may prove to be a recurring motif in disulfide-rich peptides from marine organisms.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk acrorhagin I NMR disulfides hairpin structure metal ion interaction sea anemone
Megjelenés:	Journal Of Structural Biology. - 213 : 2 (2021), p. 1-37. -
További szerzők:	Sunanda, Punnepalli Villegas-Moreno, Jessica Csóti Ágota (1989-) (biológus) Morales, Rodrigo A. V. Wai, Dorothy C. C. Panyi György (1966-) (biofizikus) Prentis, Peter Norton, Raymond S.
Pályázati támogatás:	K119417 OTKA EFOP-3.6.1-16-2016-00022 EFOP GINOP-2.3.2-15-2016-00015 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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