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001-es BibID:	BIBFORM091923
Első szerző:	Kaňa, Radek
Cím:	Fast Diffusion of the Unassembled PetC1-GFP Protein in the Cyanobacterial Thylakoid Membrane / Radek Kaňa, Gábor Steinbach, Roman Sobotka, György Vámosi, Josef Komenda
Dátum:	2020
ISSN:	2075-1729
Megjegyzések:	Biological membranes were originally described as a fluid mosaic with uniform distribution of proteins and lipids. Later, heterogeneous membrane areas were found in many membrane systems including cyanobacterial thylakoids. In fact, cyanobacterial pigment?protein complexes (photosystems, phycobilisomes) form a heterogeneous mosaic of thylakoid membrane microdomains (MDs) restricting protein mobility. The trafficking of membrane proteins is one of the key factors for long-term survival under stress conditions, for instance during exposure to photoinhibitory light conditions. However, the mobility of unbound ♭free' proteins in thylakoid membrane is poorly characterized. In this work, we assessed the maximal diffusional ability of a small, unbound thylakoid membrane protein by semi-single molecule FCS (fluorescence correlation spectroscopy) method in the cyanobacterium Synechocystis sp. PCC6803. We utilized a GFP-tagged variant of the cytochrome b6f subunit PetC1 (PetC1-GFP), which was not assembled in the b6f complex due to the presence of the tag. Subsequent FCS measurements have identified a very fast diffusion of the PetC1-GFP protein in the thylakoid membrane (D = 0.14 ?? 2.95 m2s??1). This means that the mobility of PetC1-GFP was comparable with that of free lipids and was 50?500 times higher in comparison to the mobility of proteins (e.g., IsiA, LHCII?light-harvesting complexes of PSII) naturally associated with larger thylakoid membrane complexes like photosystems. Our results thus demonstrate the ability of free thylakoid-membrane proteins to move very fast, revealing the crucial role of protein?protein interactions in the mobility restrictions for large thylakoid protein complexes.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk proteins mobility photosynthesis FCS thylakoids cyanobacteria
Megjelenés:	Life. - 11 : 1 (2020), p. 1-12. -
További szerzők:	Steinbach Gábor Sobotka, Roman Vámosi György (1967-) (biofizikus) Komenda, Josef
Pályázati támogatás:	GINOP-2.3.2- 15-2016-00001 GINOP NKFIH NN 129371 Egyéb NKFIH ANN 135107 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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