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001-es BibID:	BIBFORM092555
Első szerző:	Pintye Alexandra
Cím:	Improved DNA extraction and quantitative real-time PCR for genotyping Erysiphe necator and detecting the DMI fungicide resistance marker A495T, using single ascocarps / Alexandra Pintye, Márk Z. Németh, Orsolya Molnár, Áron N. Horváth, Zsolt Spitzmüller, Nikoletta Szalóki, Károly Pál, Kálmán Z. Váczy, Gábor M. Kovács
Dátum:	2020
ISSN:	0031-9465 1593-2095
Megjegyzések:	DNA extraction from minute fungal samples is challenging in all genetic studies. Identification of genetic groups and population biology mostly rely on the laborious production of single conidium isolates or on field samples, including infect- ed plant materials. This paper reports a simple and cost-effective protocol for DNA extraction from individual chasmothecia of Erysiphe necator for subsequent applica- tions. It is a less laborious alternative for genotyping purposes than production and analysis of single conidium isolates or analysis of infected plant material from the field. Using the protocols described here for 186 E. necator samples tested, genetic groups A and B were assigned. Based on CYP51 sequences, all the samples belonged to group B, while TUB2 sequences exhibited SNPs also diagnostic for group A. Additionally, a quantitative real-time PCR detection method of single nucleotide polymorphism in the CYP51 gene associated with DMI fungicide resistance was applied. The A495T marker, associated with DMI resistance, and here reported for the first time from Hungary, was detected by quantitative real-time PCR assays and direct sequencing of CYP51 . The methods developed in this study can be applied as routine tests to monitor powdery mildew populations for fungicide resistance and other genetic characteristics.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Phytopathologia Mediterranea. - 59 : 1 (2020), p. 97-106. -
További szerzők:	Németh Márk Z. Molnár Orsolya Horváth Áron N. Spitzmüller Zsolt (1986-) (molekuláris biológus) Szalóki Nikoletta (1981-) (biológus) Pál Károly (1978-) (mikrobiológus) Váczy Kálmán Zoltán Kovács Gábor M.
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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