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001-es BibID:	BIBFORM092908
Első szerző:	Luo, Jane
Cím:	Rapid Adeno-Associated Virus Genome Integrity Analysis by Capillary Gel Electrophoresis / Jane Luo, András Guttman
Dátum:	2020
ISSN:	1471-6577
Megjegyzések:	Adeno-associated virus (AAV) is a propitious gene therapy vector to be translated to the clinical practice and pursued accordingly by the biopharmaceutical industry. However, genome integrity and nucleic acid impurities in the final product may represent a problem with the therapeutic use. In this paper, two AAV genome integrity analysis workflows are introduced. The standard sample preparation protocol (~2.5 h) included benzonase treatment to degrade any nucleic acids outside of the AAV capsid, which were then filtered out. The residual AAV capsids were digested by proteinase K, followed by a DNA purification process involving guanidine?hydrochloride. The accelerated protocol (~15 min) utilized the DNA purification step only. In both workflows, the released DNA was analyzed by capillary gel electrophoresis with a laser induced fluorescent detection (CGE?LIF) in less than 15 min with ? 0.33% RSD on migration time and ? 2.06% RSD on peak area reproducibility.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	LC GC Europe. - 33 : 10 (2020), p. 33-38. -
További szerzők:	Guttman András (1954-) (vegyészmérnök)
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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