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001-es BibID:	BIBFORM095719
035-os BibID:	(WoS)000668898600016
Első szerző:	Shaw, Abhirup
Cím:	Mitophagy is downregulated upon thermogenic stimulus in human beige adipocytes / Shaw Abhirup, Szatmári-Tóth Mária, Csomós István, Mocsár Gábor, Balajthy Zoltán, Győry Ferenc, Kristóf Endre Károly, Fésüs László
Dátum:	2021
ISSN:	2211-5463
Megjegyzések:	Adipocytes are classi?ed into white, brown and beige. Brown and beige adipocytes are important targets to combat obesity, as they are capable to dissipate energy in the form of heat, while the white adipocytes are primarily for energy storage. UCP1, an inner mitochondrial membrane protein mediates thermogenesis by uncoupling the mitochondrial respiratory chain from ATP synthesis. Hence, mitochondria are important for the thermogenic and metabolic functions of adipocytes. UCP1+ mitochondria in human adipocytes are mostly fragmented (Pisani et al, 2017). Mitophagy plays a vital role in beige to white adipocyte transition in mouse (Altshuler-Keylin et al, 2016). We intend to characterize the role of mitophagy in the thermogenic activation of primary human abdominal subcutaneous adipocytes and SGBS cells. Isolated preadipocytes were differentiated into white and browning adipocytes, which were treated with dibutyryl-cAMP (6, 10 and 14 hours). Genes related to parkin-dependent and independent mitophagy were downregulated upon thermogenic stimulus; the parkin dependent ones being the most downregulated. The lipidated form of LC3, LC3-II is recruited on the outer membrane of the autophagosome which indicates ongoing autophagy. TOM20 is an outer mitochondrial membrane protein and marks the mitochondria. LC3 and TOM20 immunostaining were performed, followed by quanti?cation of LC3 punctae,which was high in untreated control adipocytes but decreased signi?cantly upon thermogenic stimulus, suggesting repressed autophagy/mitophagy. Colocalization of TOM20 and LC3 can indicate mitophagy. Decreased colocalization was observed upon thermogenic stimulus, which further proved repressed mitophagy.TOM20 quanti?cation showed increased number of fragmented mitochondria upon thermogenic stimulus suggesting prompt inhibition of mitophagy, thereby protecting many fragmented mitochondria from degradation and boosting thermogenesis.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idézhető absztrakt folyóiratcikk
Megjelenés:	FEBS Open Bio. - 11 : S1 (2021), p. 9. -
További szerzők:	Szatmári-Tóth Mária (1987-) (molekuláris biológus) Csomós István (1983-) (molekuláris biológus) Mocsár Gábor (1981-) (biofizikus) Balajthy Zoltán (1957-) (biokémikus, sejtbiológus) Győry Ferenc (1964-) (sebész) Kristóf Endre (1987-) (általános orvos) Fésüs László (1947-) (orvos biokémikus)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00006 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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