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001-es BibID:	BIBFORM096085
035-os BibID:	(WoS)000688514700002 (Scopus)85108233557 (PubMed)34089737
Első szerző:	Horváth Balázs (élettanász)
Cím:	Ion current profiles in canine ventricular myocytes obtained by the "onion peeling" technique / Horváth Balázs, Kiss Dénes, Dienes Csaba, Hézső Tamás, Kovács Zsigmond, Szentandrássy Norbert, Almássy János, Magyar János, Bányász Tamás, Nánási Péter P.
Dátum:	2021
ISSN:	0022-2828
Megjegyzések:	The profiles of ion currents during the cardiac action potential can be visualized by the action potential voltage clamp technique. To obtain multiple ion current data from the same cell, the "onion peeling" technique, based on sequential pharmacological dissection of ion currents, has to be applied. Combination of the two methods allows recording of several ion current profiles from the same myocyte under largely physiological conditions. Using this approach, we have studied the densities and integrals of the major cardiac inward (ICa, INCX, INa-late) and outward (IKr, IKs, IK1) currents in canine ventricular cells and studied the correlation between them. For this purpose, canine ventricular cardiomyocytes were chosen because their electrophysiological properties are similar to those of human ones. Significant positive correlation was observed between the density and integral of ICa and IKr, and positive correlation was found also between the integral of ICa and INCX. No further correlations were detected. The Ca2+-sensitivity of K+ currents was studied by comparing their parameters in the case of normal calcium homeostasis and following blockade of ICa. Out of the three K+ currents studied, only IKs was Ca2+-sensitive. The density and integral of IKs was significantly greater, while its time-to-peak value was shorter at normal Ca2+ cycling than following ICa blockade. No differences were detected for IKr or IK1 in this regard. Present results indicate that the positive correlation between ICa and IKr prominently contribute to the balance between inward and outward fluxes during the action potential plateau in canine myocytes. The results also suggest that the profiles of cardiac ion currents have to be studied under physiological conditions, since their behavior may strongly be influenced by the intracellular Ca2+ homeostasis and the applied membrane potential protocol.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk cardiac ion currents dog myocytes action potential voltage clamp sequential dissection of ionic currents onion peeling technique Ca2+ sensitive ion currents
Megjelenés:	Journal Of Molecular And Cellular Cardiology. - 158 (2021), p. 153-162. -
További szerzők:	Kiss Dénes Zsolt (1995-) (orvos, élettanász) Dienes Csaba (1995-) (gyógyszerész) Hézső Tamás (1993-) (élettanász) Kovács Zsigmond Máté (1995-) (orvos) Szentandrássy Norbert (1976-) (élettanász) Almássy János (1981-) (élettanász, biológus, angol-magyar szakfordító) Magyar János (1961-) (élettanász) Bányász Tamás (1960-) (élettanász) Nánási Péter Pál (1956-) (élettanász)
Pályázati támogatás:	FK128116 OTKA PD120794 NKFIH FK128116 NKFIH GINOP-2.3.2.-15-2016-00040 GINOP ÚNKP-20-2 Egyéb ÚNKP-20-3 Egyéb EFOP-3.6.3-VEKOP-16-2017-00009 EFOP
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