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001-es BibID:	BIBFORM096265
035-os BibID:	(WoS)000465562600014 (Scopus)85064411777
Első szerző:	Ditrói Tamás (vegyész)
Cím:	Comprehensive analysis of how experimental parameters affect H2S measurements by the monobromobimane method / Tamás Ditrói, Attila Nagy, Diego Martinelli, András Rosta, Viktor Kožich, Péter Nagy
Dátum:	2019
ISSN:	0891-5849
Megjegyzések:	A rapidly increasing number of studies report on widespread biological functions for endogenous hydrogen sulfide. However, the use of multiple, chemically distinct analytical methods to measure free hydrogen sulfide levels in biological samples accumulate data that are not in agreement with each other. In this work a widely appreciated technique, the monobromobimane method, was thoroughly investigated with the overall aims i) to demonstrate how results obtained by different versions of the method should be interpreted and ii) to provide an easy protocol for the community in order to obtain reliable and comparable results. We demonstrate that none of the previously published versions of the method measure free sulfide concentrations in blood serum or plasma samples due to significant interferences with the biomolecule-bound sulfide pool. On the other hand, we stress the biological relevance of these measurements in cases in which they are carefully conducted. To aid future studies, we extensively investigated the entire procedure from sample withdrawal through handling and storing of injection-ready samples until the detection protocol in order to pinpoint all parameters that can affect the final readouts. Based on our rigorous analytical investigations a set of recommendations were compiled that are necessary to ensure reliable, reproducible and comparable results in the field and a detailed standardized pro tocol is provided.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Hydrogen sulfide Monobromobimane method Sulfide pool Blood sulfide levels
Megjelenés:	Free Radical Biology And Medicine. - 136 (2019), p. 146-158. -
További szerzők:	Nagy Attila Martinelli, Diego Rosta András (onkológus Budapest) Kožich, Viktor Nagy Péter (1976-) (vegyész)
Pályázati támogatás:	K 109843 OTKA KH17_126766 Egyéb K 129286 OTKA NVKP_16-1-2016-0005 Egyéb GINOP-2.3.2-15-2016-00043 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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