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001-es BibID:	BIBFORM098753
035-os BibID:	(cikkazonosító)11514 (scopus)85117957430 (wos)000722332400001
Első szerző:	Vörös Orsolya (biotechnológus)
Cím:	Immune Synapse Residency of Orai1 Alters Ca2+ Response of T Cells / Vörös Orsolya, Panyi György, Hajdu Péter
Dátum:	2021
ISSN:	1661-6596 1422-0067
Megjegyzések:	Abstract CRAC, which plays important role in Ca2+-dependent T-lymphocyte activation, is composed of the ER-resident STIM1 and the plasma membrane Orai1 pore-forming subunit. Both accumulate at the immunological synapse (IS) between a T cell and an antigen-presenting cell (APC). We hypothesized that adapter/interacting proteins regulate Orai1 residence in the IS. We could show that mGFP-tagged Orai1-Full channels expressed in Jurkat cells had a biphasic IS-accumulation kinetics peaked at 15 min. To understand the background of Orai1 IS-redistribution we knocked down STIM1 and SAP97 (adaptor protein with a short IS-residency (15 min) and ability to bind Orai1 N-terminus): the mGFP-Orai1-Full channels kept on accumulating in the IS up to the 60th minute in the STIM1- and SAP97-lacking Jurkat cells. Deletion of Orai1 N terminus (mGFP-Orai1-?72) resulted in the same time course as described for STIM1/SAP97 knock-down cells. Ca2+-imaging of IS-engaged T-cells revealed that of Orai1 residency modifies the Ca2+-response: cells expressing mGFP-Orai1-?72 construct or mGFP-Orai1-Full in SAP-97 knock-down cells showed higher number of Ca2+-oscillation up to the 90th minute after IS formation. Overall, these data suggest that SAP97 may contribute to the short-lived IS-residency of Orai1 and binding of STIM1 to Orai1 N-terminus is necessary for SAP97-Orai1 interaction.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Orai1 immunological synapse calcium signaling STIM1 SAP97
Megjelenés:	International Journal Of Molecular Sciences. - 22 : 21 (2021), p. 1-19. -
További szerzők:	Panyi György (1966-) (biofizikus) Hajdu Péter (1975-) (biofizikus)
Pályázati támogatás:	K128525 NKFIH GINOP-2.3.2-15-2016-00044 GINOP K119417 NKFIH EFOP-3.6.2-16-2017-00006 EFOP EFOP-3.6.3-VEKOP-16-2017-00009 EFOP GINOP-2.3.2-15-2016-00020 GINOP
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