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001-es BibID:	BIBFORM100024
035-os BibID:	(cikkazonosító)1319 (scopus)85123310479 (wos)000755993200001
Első szerző:	Pierantozzi, Enrico
Cím:	Impaired Intracellular Ca2+ Dynamics, M-Band and Sarcomere Fragility in Skeletal Muscles of Obscurin KO Mice / Pierantozzi Enrico, Szentesi Péter, Paolini Cecilia, Dienes Beatrix, Fodor János, Oláh Tamás, Colombini Barbara, Rassier Dilson E., Rubino Egidio Maria, Lange Stephan, Rossi Daniela, Csernoch László, Bagni Maria Angela, Reggiani Carlo, Sorrentino Vincenzo
Dátum:	2022
ISSN:	1661-6596 1422-0067
Megjegyzések:	Obscurin is a giant sarcomeric protein expressed in striated muscles known to establish several interactions with other proteins of the sarcomere, but also with proteins of the sarcoplasmic reticulum and costameres. Here, we report experiments aiming to better understand the contribution of obscurin to skeletal muscle fibers, starting with a detailed characterization of the diaphragm muscle function, which we previously reported to be the most affected muscle in obscurin (Obscn) KO mice. Twitch and tetanus tension were not significantly different in the diaphragm of WT and Obscn KO mice, while the time to peak (TTP) and half relaxation time (HRT) were prolonged. Differences in force-frequency and force-velocity relationships and an enhanced fatigability are observed in an Obscn KO diaphragm with respect to WT controls. Voltage clamp experiments show that a sarcoplasmic reticulum's Ca2+ release and SERCA reuptake rates were decreased in muscle fibers from Obscn KO mice, suggesting that an impairment in intracellular Ca2+ dynamics could explain the observed differences in the TTP and HRT in the diaphragm. In partial contrast with previous observations, Obscn KO mice show a normal exercise tolerance, but fiber damage, the altered sarcomere ultrastructure and M-band disarray are still observed after intense exercise.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk obscurin skeletal muscle calcium dynamics muscle fiber damage kinetics of contraction sarcoplasmic reticulum exercise
Megjelenés:	International Journal Of Molecular Sciences. - 23 : 3 (2022), p. 1319. -
További szerzők:	Szentesi Péter (1967-) (élettanász) Paolini, Cecilia Dienes Beatrix (1972-) (élettanász, molekuláris biológus) Fodor János (1973-) (élettanász, biotechnológus) Oláh Tamás (1983-) (élettanász) Colombini, Barbara Rassier, Dilson E. Rubino, Egidio Maria Lange, Stephan Rossi, Daniela Csernoch László (1961-) (élettanász) Bagni, Maria Angela Reggiani, Carlo Sorrentino, Vincenzo
Pályázati támogatás:	K 137600 OTKA TKP2020-NKA-04 Egyéb 2020-4.1.1-TKP2020 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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