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001-es BibID:	BIBFORM102019
035-os BibID:	(WoS)000803644300001 (Scopus)85130408566
Első szerző:	Bécsi Bálint (vegyészmérnök)
Cím:	Epigallocatechine-3-gallate Inhibits the Adipogenesis of Human Mesenchymal Stem Cells via the Regulation of Protein Phosphatase-2A and Myosin Phosphatase / Bálint Bécsi, Zoltán Kónya, Anita Boratkó, Katalin Kovács, Ferenc Erdődi
Dátum:	2022
ISSN:	2073-4409
Megjegyzések:	Epigallocatechin-3-gallate (EGCG) has widespread effects on adipocyte development. However, the molecular mechanisms of EGCG are not fully understood. We investigate the adipogenic differentiation of human-derived mesenchymal stem cells, including lipid deposition and changes in the expression and phosphorylation of key transcription factors, myosin, protein phosphatase-2A (PP2A), and myosin phosphatase (MP). On day 6 of adipogenic differentiation, EGCG (1-20 ?M) suppressed lipid droplet formation, which was counteracted by an EGCG-binding peptide for the 67 kDa laminin receptor (67LR), suggesting that EGCG acts via 67LR. EGCG decreased the phosphorylation of CCAAT-enhancer-binding protein beta via the activation of PP2A in a protein kinase A (PKA)-dependent manner, leading to the partial suppression of peroxisome proliferator-activated receptor gamma (PPARγ) and adiponectin expression. Differentiated cells exhibited a rounded shape, cortical actin filaments, and lipid accumulation. The EGCG treatment induced cell elongation, stress fiber formation, and less lipid accumulation. These effects were accompanied by the degradation of the MP target subunit-1 and increased the phosphorylation of the 20 kDa myosin light chain. Our results suggest that EGCG acts as an agonist of 67LR to inhibit adipogenesis via the activation of PP2A and suppression of MP. These events are coupled with the decreased phosphorylation and expression levels of adipogenic transcription factors and changes in cell shape, culminating in curtailed adipogenesis.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk 67 kDa laminin receptor (67LR) adipogenesis epigallocatechin-3-gallate (EGCG) mesenchymal stem cells myosin phosphatase protein phosphatase-2A (PP2A)
Megjelenés:	Cells. - 11 : 10 (2022), p. 1-18. -
További szerzők:	Kónya Zoltán (1986-) (molekuláris biológus, biokémikus) Boratkó Anita (1985-) (biokémikus, molekuláris biológus) Kovács Katalin (1978-) (biokémikus) Erdődi Ferenc (1953-) (biokémikus)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00044 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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