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001-es BibID:	BIBFORM103631
035-os BibID:	(cikkazonosító)418 (Scopus)85138644430
Első szerző:	Nath, Arijit
Cím:	Hydrolysis of Soybean Milk Protein by Papain : antioxidant, Anti-Angiotensin, Antigenic and Digestibility Perspectives / Nath Arijit, Ahmad Abubakar Saleh, Amankwaa Abraham, Csehi Barbara, Mednyánszky Zsuzsanna, Szerdahelyi Emőke, Tóth Attila, Tormási Judit, Truong Duy Hoang, Abrankó László, Koris András
Dátum:	2022
ISSN:	2306-5354
Megjegyzések:	Abstract: The objective of the investigation was to understand the biochemical activities of hy- drolysate of soybean milk protein (SMP). Hydrolysis was carried out by different concentrations of papain (0.008 g?L?1, 0.016 g?L?1, 0.032 g?L?1 and 0.064 g?L?1). The antioxidant capacity was measured by the ferric-reducing ability of plasma (FRAP) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assays. The anti-angiotensin activity of hydrolysate was measured by the recombinant angiotensin converting enzyme and substrate Abz-FRK(Dnp)-P. The contributions of the Kunitz trypsin in- hibitor (KTI) and Bowman?Birk inhibitor (BBI) on antigenicity, and the in vitro digestion of papain- hydrolyzed SMP were studied. Rabbit polyclonal anti-KTI and anti-BBI antibodies together with peroxidase-labelled goat anti-Rb IgG secondary antibody were used to identify the antigenicity of KTI and BBI in unhydrolyzed and papain-hydrolyzed SMP. The antioxidant capacity and anti-angiotensin activity of SMP were increased after the papain hydrolysis of SMP. The KTI- and BBI-specific antigenic- ity were reduced in SMP by increasing the concentration of papain. However, there was interaction between papain-hydrolyzed SMP and trypsin in native gel, while interaction with chymotrypsin was absent. The interaction between trypsin and SMP was reduced due to the hydrolysis of papain in a concentration-dependent manner. According to the in vitro gastrointestinal digestion simulation protocol (Infogest), the digestibility of SMP was not statistically increased.
Tárgyszavak:	Agrártudományok Élelmiszertudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Bioengineering. - 9 : 9 (2022), p. 1-21. -
További szerzők:	Ahmad, Abubakar Saleh Amankwaa, Abraham Csehi Barbara Mednyánszky Zsuzsanna Szerdahelyi Emőke Tóth Attila (1971-) (biológus) Tormási Judit Truong, Duy Hoang Abrankó László Koris András
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