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001-es BibID:	BIBFORM103651
035-os BibID:	(cikkazonosító)109184 (scopus)85128192258 (wos)000793580100003
Első szerző:	Imre László (biológus)
Cím:	Nucleosome destabilization by polyamines / Imre Laszlo, Niaki Erfaneh Firouzi, Bosire Rosevalentine, Nanasi Peter Jr., Nagy Peter, Bacso Zsolt, Hamidova Nubar, Pommier Yves, Jordan Albert, Szabo Gabor
Dátum:	2022
ISSN:	0003-9861
Megjegyzések:	The roles and molecular interactions of polyamines (PAs) in the nucleus are not fully understood. Here their effect on nucleosome stability, a key regulatory factor in eukaryotic gene control, is reported, as measured in agarose embedded nuclei of H2B-GFP expressor HeLa cells. Nucleosome stability was assessed by quantitative microscopy [1,2] in situ, in close to native state of chromatin, preserving the nucleosome constrained topology of the genomic DNA. A robust destabilizing effect was observed in the millimolar concentration range in the case of spermine, spermidine as well as putrescine, which was strongly pH and salt concentration-dependent, and remained significant also at neutral pH. The integrity of genomic DNA was not affected by PA treatment, excluding DNA break-elicited topological relaxation as a factor in destabilization. The binding of PAs to DNA was demonstrated by the displacement of ethidium bromide, both from deproteinized nuclear halos and from plasmid DNA. The possibility that DNA methylation patterns may be influenced by PA levels is contemplated in the context of gene expression and DNA methylation correlations identified in the NCI-60 panel-based CellMiner database: methylated loci in subsets of high-ODC1 cell lines and the dependence of PER3 DNA methylation on PA metabolism.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Archives Of Biochemistry And Biophysics. - 722 (2022), p. 1-11. -
További szerzők:	Niaki, Erfaneh Firouzi Bosire, Rosevalentine (1988-) (biotechnológus) Nánási Péter Pál ifj. (1987-) (sejtbiológus) Nagy Péter (1971-) (biofizikus) Bacsó Zsolt (1963-) (biofizikus) Hamidova, Nubar Pommier, Yves Jordan, Albert Szabó Gábor (1953-) (biofizikus)
Pályázati támogatás:	K128770 OTKA K138524 OTKA COST Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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