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001-es BibID:	BIBFORM104499
Első szerző:	Astuti, Putri Kusuma (állattenyésztési genetikus)
Cím:	Polymorphism of genes associated with immune response in French Alpine and Saanen in Hungary / Putri Kusuma Astuti, George Wanjala, Sándor Kukovics, Szilvia Kusza
Dátum:	2022
Megjegyzések:	Interleukin (IL) is a cytokine found in animals that has a role in the induction and regulation of the immune response. Numerous publications have investigated IL genes in livestock, but little research has been conducted on goats. In goats, IL has a critical role in response to disease infections such as Haemonchus contortus and Clostridium perfringens. This study aimed to investigate the polymorphism of three IL genes located in three chromosomes: rs648293427- Interleukin 15 Receptor Subunit Alpha (L15RA), rs659842900- Interleukin 1 Receptor Antagonist (IL1RN), and rs667413402- Interleukin-8 (IL8) in Hungarian French Alpine (n=19) and Saanen (n=25) goats using the Kompetitive Allele-Specific Polymerase Chain Reaction (KASP-PCR) technique. The frequencies of alleles and genotypes, fixation index (Fis), observed heterozygosity, expected heterozygosity, F-statistic (Fst), and their agreement with or divergence from Hardy?Weinberg equilibrium (HWE) were determined using POPGENEversion 1.31 software, as well as the polymorphic information content (PIC) through online website https://gene-calc.pl/pic. Similar genotypic and allelic frequencies were observed in the two populations, except for rs648293427-IL15RA. Allele C frequency in French Alpine populations (0.842) was higher than in the Saanen population (0.580). Furthermore, the homozygote CC was mainly observed in French Alpine population and heterozygote TC in Saanen populations. The rs648293427-IL15RA SNP was the most polymorphic marker based on the PIC value. It also showed the highest observed heterozygosity (0.440) value, which indicated a high within- population diversity in the Saanen population. A close relationship between the two populations was observed through the low Fst value (0.008-0.084). All observed SNPs in this study adhered to HWE. This study validated three polymorphic SNPs in two Hungarian breeds, which can be utilized as markers in selecting immunity in Hungarian goats.
ISBN:	978-615-01-3419-2
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok előadáskivonat könyvrészlet Polymorphism goats Interleukin KASP-PCR
Megjelenés:	13th International Conference on Goats "ICG 2022": Book of Abstracts - Goats for the Future - Goat production in a changing environment / eds. Lucia Sepe, Carina Visser, Noemí Castro, Paula Menzies, Anastasio Argüello, Sándor Kukovics. - p. 28. -
További szerzők:	Wanjala, George (1984-) (Animal scientist) Kukovics Sándor (1950-) (agrár) Kusza Szilvia (1979-) (agrármérnök)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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