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001-es BibID:	BIBFORM105350
035-os BibID:	(cikkazonosító)108736 (WoS)000889935000011 (Scopus)85141915238
Első szerző:	Mutuku, Christopher
Cím:	Plasmid sequence dataset of multidrug-resistant Enterobacterales isolated from hospital effluents and wastewater treatment plant / Christopher Mutuku, Barbara Kutasy, Peter Urban, Szilvia Melegh, Robert Herczeg, Zoltan Gazdag, Eszter Virág
Dátum:	2022
ISSN:	2352-3409
Megjegyzések:	We present plasmid sequences of 21 multidrug resistant isolates of Enterobacterales belonging to Escherichia coli (n=10), Klebsiella pneumoniae (n=9), Klebsiella oxytoca (n=1), and Citrobacter freundii (n=1). The isolates originated from effluent collected from hospital sewer pipes and from a wastewater treatment plant (WWTP) in a southwestern Hungarian city. Isolation was carried out using eosin methylene blue agar supplemented with ceftriaxone and the isolates were identified with MALDI-TOF MS. Screening for multidrug resistance was conducted by determining susceptibility to four chemical classes namely, beta-lactams, aminoglycoside, fluoroquinolone, and sulfonamide. Plasmid DNA was isolated by alkaline lysis method using the Monarch plasmid DNA miniprep kit from freshly grown pure colonies. Molecular typing and Illumina sequencing of plasmid DNA of multiresistant strains were performed. After the assembly of contigs, genes localized on plasmid sequences were determined and functionally annotated. These reconstructed plasmid sequences supplemented with gene functional annotations were deposited in the Mendeley data. Using these datasets different plasmid incompatibility groups were identified. These conjugative plasmids appear to play a key role in the transmission of multiple resistance genes in enteric bacteria via wastewater. The presented data may provide useful insight on the correlations between environmental antibiotic contamination and the development of bacterial resistance, which poses a serious public health threat.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Hospital effluents Wastewater treatment plant Enterobacterales Beta-lactamases Resistance genes Plasmid antibiotic resistance
Megjelenés:	Data in Brief. - 45 (2022), p. 1-9. -
További szerzők:	Kutasy Barbara Urbán Péter Melegh Szilvia Herczeg Róbert Gazdag Zoltán Virág Eszter (1982-) (biológus)
Pályázati támogatás:	PTE Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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