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001-es BibID:BIBFORM105734
035-os BibID:(WOS)000319074600029 (Scopus)84878011489
Első szerző:Siesto, Gabriella
Cím:Polymorphism detection among wild Saccharomyces cerevisiae strains of different wine origin / Gabriella Siesto, Angela Capece, Matthias Sipiczki, Hajnalka Csoma, Patrizia Romano
Dátum:2013
ISSN:1590-4261 1869-2044
Megjegyzések:In this study, wild Saccharomyces cerevisiae strains, isolated from spontaneously fermenting grapes of different varieties and origins, were submitted to genetic analysis using different molecular techniques, such as amplification of genes coding for cell wall proteins and containing minisatellite-like sequences, karyotyping, mtDNA-RFLP, and analysis of the delta region. The lowest discriminative power was obtained by minisatellites analysis, in particular the amplification of AGA1 genes. Karyotyping and mtDNA-RFLP analysis yielded the same differentiation among the strains, whereas the PCR amplification of delta sequences resulted the best method as it was fast and it showed a very high discriminative power. In any case, it has to be underlined that some strains, showing the same delta profiles, exhibited a different mtDNA restriction profile and electrophoretic karyotype, suggesting that more than one molecular marker is required for reliable strain discrimination. Although the techniques used revealed a different resolution power, they all revealed a genetic relationship among strains isolated from spontaneous fermentation of grapes of different origins. In fact, none of the typing methods was able to discriminate some strains isolated from different areas.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Saccharomyces cerevisiae
Wine strains
Minisatellites
Karyotyping
mtDNA-RFLP
Interdelta region
Megjelenés:Annals of Microbiology. - 63 : 2 (2013), p. 661-668. -
További szerzők:Capece, Angela Sipiczki Mátyás (1948-) (biológus) Csoma Hajnalka (1979-) (biológus, szőlész-borász) Romano, Patrizia
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