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001-es BibID:	BIBFORM106841
035-os BibID:	(cikkazonosító)112347 (WoS)000921144100001 (Scopus)85145252534
Első szerző:	Gonda Sándor (gyógyszerész)
Cím:	Quality-controlled LC-ESI-MS food metabolomics of fenugreek (Trigonella foenum-graecum) sprouts: Insights into changes in primary and specialized metabolites / Sándor Gonda, Zsolt Szűcs, Tamás Plaszkó, Zoltán Cziáky, Attila Kiss-Szikszai, Dávid Sinka, Ildikó Bácskay, Gábor Vasas
Dátum:	2023
ISSN:	0963-9969
Megjegyzések:	Fenugreek (Trigonella foenum-graecum L.) is an important food and spice with bioactive compounds against diabetes. In this study, fenugreek seeds germinating in darkness for 72 h were studied using quantification of trigonelline and 4-hydroxyisoleucine and an LC-ESI-MS/MS-based metabolomic approach capable of accurately estimating 237 features from various primary and specialized compound classes. During germination, the concentrations of trigonelline and 4-hydroxyisoleucine rose by 33.5% and 33.3%, respectively. At the same time, untargeted metabolomics revealed 9 putative flavonoids increasing 1.19-to 2.77 -fold compared to the dormant seeds. A set of 19 steroid saponins rose by 1.08-to 31.86-fold. Primary metabolites however showed much more variability: abundance changes in amino acid derivatives, peptides and saccharides fell in the 0.09-to 22.25-fold, 0.93-to 478.79-fold and 0.36-to 941.58-fold ranges, respectively. To increase biosynthesis of specialized metabolites during germination, sprouts were exposed to 1-100 mM methyl jasmonate (MeJA) and methyl salicylate (MeSA). The hormone treatments affected normal metabolism: 67.1-83.1 % and 64.1-83.5 % of compounds showed a reduction compared to the controls in 100 mM MeJA and MeSA treatments at different sampling time points. Contrary to expectations, the abundance of flavonoids decreased, compared to the control sprouts (0.75-and 0.68-fold change medians, respectively). The same was observed for most, but not all steroid saponins. The quality-controlled untargeted metabolomics approach proved to yield excellent insight into the metabolic changes during germination of fenugreek. The results suggest that although fenugreek germination causes major shifts in plant metabolism, there are no major qualitative changes in bioactive specialized metabolites during the first three days. This stability likely translates into good bioactivity that is similar to that of the seeds. Because the large changes in the primary metabolites likely alter the nutritive value of the seed, further studies are warranted.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Food Research International. - 164 (2023), p. 1-13. -
További szerzők:	Szűcs Zsolt (1990-) (gyógyszerész) Plaszkó Tamás (1994-) (Biológus Msc) Cziáky Zoltán Kiss-Szikszai Attila (1975-) (vegyész, műszeres-analitikus szakvegyész) Sinka Dávid Zsolt (1991-) (gyógyszerész) Bácskay Ildikó (1969-) (gyógyszerész, gyógyszertechnológus) Vasas Gábor (1975-) (biológus-vegyész)
Pályázati támogatás:	OTKA-128021 OTKA TKP2020-IKA-04 Egyéb GINOP-2.2.1-15-2017-00066 GINOP GINOP-2.3.3-15-2016-00021 GINOP ÚNKP-22-3-II-DE-128 Egyéb
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