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001-es BibID:	BIBFORM107426
035-os BibID:	(WoS)000598288900001 (Scopus)85096973030
Első szerző:	Majdoub, Saida
Cím:	Concentration-Dependent Interactions of Amphiphilic PiB Derivative Metal Complexes with Amyloid Peptides A beta and Amylin** / Saida Majdoub, Zoltán Garda, Alexandre C. Oliveira, Inga Relich, Agnès Pallier, Sara Lacerda, Christelle Hureau, Carlos F.G.C. Geraldes, Jean-François Morfin, Éva Tóth
Dátum:	2020
ISSN:	0947-6539 1521-3765
Megjegyzések:	Metal chelates targeted to amyloid peptides are widely explored as diagnostic tools or therapeutic agents. The attachment of a metal complex to amyloid recognition units typically leads to a decrease in peptide affinity. We show here that by separating a macrocyclic GdL chelate and a PiB targeting unit with a long hydrophobic C10 linker, it is possible to attain nanomolar affinities for both A beta(1-40) (K-d=4.4 nm) and amylin (K-d=4.5 nm), implicated, respectively in Alzheimer's disease and diabetes. The Scatchard analysis of surface plasmon resonance data obtained for a series of amphiphilic, PiB derivative GdL complexes indicate that their A beta(1-40) or amylin binding affinity varies with their concentration, thus micellar aggregation state. The GdL chelates also affect peptide aggregation kinetics, as probed by thioflavin-T fluorescence assays. A 2D NMR study allowed identifying that the hydrophilic region of A beta(1-40) is involved in the interaction between the monomer peptide and the Gd3+ complex. Finally, ex vivo biodistribution experiments were conducted in healthy mice by using In-111 labeled analogues. Their pancreatic uptake, similar to 3 %ID g(-1), is promising to envisage amylin imaging in diabetic animals.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk amyloid peptide AB amylin metal complex micellar aggregation
Megjelenés:	Chemistry-A European Journal. - 27 : 6 (2020), p. 2009-2020. -
További szerzők:	Garda Zoltán (1989-) (vegyész) Oliveira, de A. C. Relich, Inga Pallier, Agnès Lacerda, Sara Hureau, Christelle Geraldes, Carlos Frederico de Gusmão Campos Morfin, Jean-François Tóth Éva
Pályázati támogatás:	DIVA ANR-16-CE18-0022-01 Egyéb UIDB/00313/2020 Egyéb FEDER/COMPETE 2020- EU Egyéb PhD grant SFRH/BD/120934/2016 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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