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001-es BibID:	BIBFORM108316
035-os BibID:	(WOS)000186399200009 (Scopus)0141532923
Első szerző:	Varga János Tamás (Budapest)
Cím:	Diversity of polyketide synthase gene sequences in Aspergillus species / János Varga, Krisztina Rigó, Sándor Kocsubé, Balázs Farkas, Károly Pál
Dátum:	2003
ISSN:	0923-2508
Megjegyzések:	Fungal polyketide synthases are responsible for the biosynthesis of several mycotoxins and other secondary metabolites. The aim of our work was to investigate the diversity of polyketide synthases in Aspergillus species using two approaches: PCR amplification using oligonucleotide primers, and bioinformatics. Ketosynthase domain probes amplified DNA fragments of about 700 bp in each examined isolate. Sequences of these domains were aligned and analyzed by phylogenetic methods. The ketosynthase domain sequences were highly diverse indicating that they most probably represent polyketide synthases responsible for different functions. A. albertensis and A. niger ketosynthase domain sequences clustered together with sequences of genes required for pigment biosynthesis (wA) in A. nidulans and P. patulum, while the ketosynthase domain sequence of A. muricatus was most closely related to an A. parasiticus wA type domain sequence, and those of the A. ochraceus isolates formed a distinct clade on the tree. These sequences were highly homologous to an A. terreus naphthopyrone synthase gene. An Aspergillus fumigatus genomic database was also searched for ketosynthase domain sequences, which have been included in the phylogenetic analysis. Altogether 14 putative ketosynthase domain sequences were identified. Clustering of the ketosynthase domain sequences correlated well with the type of metabolites produced by the corresponding polyketide synthases. At least 8 clusters with putative ketosynthase domain sequences of unknown function have been identified. Further studies are in progress to clarify the role of some of the identified polyketide synthase genes.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Aspergillus genomics ketosynthase domain mycotoxin ochratoxin phylogenetics polyketide synthase
Megjelenés:	Research In Microbiology. - 154 : 8 (2003), p. 593-600. -
További szerzők:	Rigó Krisztina Kocsubé Sándor Farkas Balázs Pál Károly (1978-) (mikrobiológus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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