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001-es BibID:	BIBFORM109149
035-os BibID:	(WOS)000170678400026 (Scopus)0034891759
Első szerző:	Prokisch József (vegyész)
Cím:	Estimation of the minimum uncertainty of DNA concentration in a genetically modified maize sample candidate certified reference material / Prokisch, J. ; Zeleny, R. ; Trapmann, S. ; Guern, L. Le ; Schimmel, H. ; Kramer, G. N. ; Pauwels, J.
Dátum:	2001
ISSN:	0937-0633
Megjegyzések:	Homogeneity testing and the determination of minimum sample mass are an important part of the certification of reference materials. The smallest theoretically achievable uncertainty of certified concentration values is limited by the concentration distribution of analyte in the different particle size fractions of powdered biological samples. This might be of special importance if the reference material is prepared by dry mixing, a dilution technique which is used for the production of the new and third generation of genetically modified (GMO) plant certified reference materials. For the production of dry mixed PMON 810 maize reference material a computer program was developed to calculate the theoretically smallest uncertainty for a selected sample intake. This model was used to compare three differently milled maize samples, and the effect of dilution on the uncertainty of the DNA content of GMO maize was estimated as well. In the case of a 50-mg sample mass the lowest achievable standard deviation was 2% for the sample containing 0.1% GMO and the minimum deviation was less than 0.5% for the sample containing 5% GMO.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Fresenius' Journal of Analytical Chemistry. - 370 : 7 (2001), p. 935-939. -
További szerzők:	Zeleny, R. Trapmann, S. Guern, L. Le Schimmel, H. Kramer, G. N. Pauwels, J.
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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