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001-es BibID:	BIBFORM110538
Első szerző:	Máthé Csaba (biológus)
Cím:	New insights into the roles of PP2A subunits FASS and C3/C4 in the regulation of mitosis and oxidative stress responses in Arabidopsis / Csaba Máthé, Zoltán Kónya, Adrienn Kelemen, Tamás Garda, Márta M-Hamvas, Gabriella Petra Juhász, Csongor Freytag
Dátum:	2023
Megjegyzések:	PP2A, a serine-threonine protein phosphatase is a complex consisting of A (scaffolding), B (regulatory) and C (catalytic) subunits. By using wild-type and loss-of-function phosphatase-related mutants (fass for a B" and c3c4 for two catalytic subunit isoforms that interact with Fass in wild-type plants) of Arabidopsis, we have found that as expected, C3/C4 subunits are responsible for a significant proportion of total PP2A activity, and surprisingly affect the activity of a related phosphatase, PP1 as well. We show for the first time that fass mutants are not impaired significantly in total phosphatase activity, because instead of decreasing PP2A, the balance between PP2A-PP1 activities is altered. Immunohistochemical studies for microtubules and phospho-histone H3 show that both types of PP2A subunits regulate mitotic activity in the root apical meristem and influence the phosphorylation of histone H3 during mitosis, a process important in the regulation of metaphase-anaphase transition and the entire mitotic process. Fass exerts this function not only by regulating PP2A (possibly involving its subcellular localization), but also by influencing PP1 activity. The two types of subunits are affecting reactive oxygen species (ROS) production (histochemical studies) and scavenging as well. By studying the activities of different superoxide dismutase (SOD) isoforms, we found that the primary target of Fass and in relation to this, C3 and C4 is Cu/Zn SOD. The protein phosphatase inhibitor microcystin-LR increased the susceptibility of mutants to both mitotic anomalies and alterations of ROS metabolism.
Tárgyszavak:	Biológiai tudományok Természettudományok előadáskivonat könyvrészlet
Megjelenés:	Botanical Microscopy Meeting 2023 / Kim Findlay, Christine Faulkner; Patrick Hussey; Ulla Neumann; Richard Smith; Imogen Sparkes. - p. 77-78.
További szerzők:	Kónya Zoltán (1986-) (molekuláris biológus, biokémikus) Kelemen Adrienn (1995-) (Biológus) Garda Tamás (1990-) (biológus) Mikóné Hamvas Márta (1963-) (biológus) Juhász Gabriella Petra (1994-) (biologia tanár) Freytag Csongor (1993-) (biológus)
Pályázati támogatás:	K120638 OTKA
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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