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001-es BibID:	BIBFORM112447
035-os BibID:	(WoS)001017061900001 (Scopus)85163667515
Első szerző:	Skopál Adrienn (gyógyszerész)
Cím:	Adenosine A2A Receptor Activation Regulates Niemann-Pick C1 Expression and Localization in Macrophages / Skopál Adrienn, Ujlaki Gyula, Gerencsér Attila Tibor, Bankó Csaba, Bacsó Zsolt, Ciruela Francisco, Virág László, Haskó György, Kókai Endre
Dátum:	2023
ISSN:	1467-3045
Megjegyzések:	Adenosine plays an important role in modulating immune cell function, particularly T cells and myeloid cells, such as macrophages and dendritic cells. Cell surface adenosine A(2A) receptors (A(2A)R) regulate the production of pro-inflammatory cytokines and chemokines, as well as the proliferation, differentiation, and migration of immune cells. In the present study, we expanded the A(2A)R interactome and provided evidence for the interaction between the receptor and the Niemann-Pick type C intracellular cholesterol transporter 1 (NPC1) protein. The NPC1 protein was identified to interact with the C-terminal tail of A(2A)R in RAW 264.7 and IPMCYRILLIC CAPITAL LETTER EF cells by two independent and parallel proteomic approaches. The interaction between the NPC1 protein and the full-length A(2A)R was further validated in HEK-293 cells that permanently express the receptor and RAW264.7 cells that endogenously express A(2A)R. A(2A)R activation reduces the expression of NPC1 mRNA and protein density in LPS-activated mouse IPMCYRILLIC CAPITAL LETTER EF cells. Additionally, stimulation of A(2A)R negatively regulates the cell surface expression of NPC1 in LPS-stimulated macrophages. Furthermore, stimulation of A(2A)R also altered the density of lysosome-associated membrane protein 2 (LAMP2) and early endosome antigen 1 (EEA1), two endosomal markers associated with the NPC1 protein. Collectively, these results suggested a putative A(2A)R-mediated regulation of NPC1 protein function in macrophages, potentially relevant for the Niemann-Pick type C disease when mutations in NPC1 protein result in the accumulation of cholesterol and other lipids in lysosomes.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Current Issues in Molecular Biology. - 45 : 6 (2023), p. 4948-4969. -
További szerzők:	Ujlaki Gyula (1991-) (molekuláris biológus) Gerencsér Attila Tibor (1999-) (orvos) Bankó Csaba (1989-) (molekuláris biológus) Bacsó Zsolt (1963-) (biofizikus) Ciruela, Francisco Virág László (1965-) (biokémikus, sejtbiológus, farmakológus) Haskó György (1967-) (biokémikus) Kókai Endre (1971-) (biokémikus, biológus)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00020 GINOP GINOP-2.3.2-15-2016-00048 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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