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001-es BibID:	BIBFORM118856
Első szerző:	Szarvas József
Cím:	Sequence analysis of partial tef1 and rpb2 genes of different Pleurotus eryngii isolates by means of PCR-RFLP / Szarvas József; Pál Károly; Geösel András
Dátum:	2014
Megjegyzések:	The Pleurotus eryngii species complex includes several varieties and certain groups with ambiguous taxonomic position. At the moment the following taxa are recognized: var. eryngii; var. ferulae /syn.: P. fuscus var. ferulae/; var. elaeoselini; var. nebrodensis; var. tingitanus; var. tuoliensis; P. hadamardii; P. fossulatus. In the course of our studies sequence analysis of the tef1á and rpb2 genes was performed, in order to distinguish varieties and reveal variability between isolates. Specific regions of the tef1á and rpb2 genes were amplified in PCR experiments. The fragments were cleaned, sequenced, aligned then BLAST searched against the NCBI GenBank nucleotide database. Some point mutations were detected in the sequences, which were used for selection of differentiating restriction enzymes for subsequent PCR-RFLP experiments. The tef1? gene sequences showed 100% identity in each strain. In contrast to that, point mutations were detected in the 21, 372 and 957 positions of the rpb2 sequences. In silico digestion was performed on the sequences and two restriction endonucleases, the BsmAI and TspDTI were selected for PCR-RFLP experiments. As a result of the digestions, the isolates could be grouped into two groups with both enzymes. At the same time, BLAST search with both amplified sequences did not give reliable information neither on varietas, nor on species level. The rpb2 locus, with its higher level of polymorphism, is a potential candidate for differentiation between varieties or identification on varietas level. Based on the results we plan to investigate which loci and molecular methods may be suitable for differentiation of isolates.
Tárgyszavak:	Agrártudományok Élelmiszertudományok előadáskivonat könyvrészlet Pleurotus eryngii tef1a rpb2 PCR-RFLP
Megjelenés:	Proceedings of 8th International Conference on Mushroom Biology and Mushroom Products Vol. 1. / ed. Manjit Singh. - p. 151-159
További szerzők:	Pál Károly (1978-) (mikrobiológus) Geösel András
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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