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001-es BibID:	BIBFORM119550
035-os BibID:	(cikkazonosító)2825 (scopus)85180451810 (wos)001131446900001
Első szerző:	Ghaffarinia, Ameneh
Cím:	Unraveling Transcriptome Profile, Epigenetic Dynamics, and Morphological Changes in Psoriasis-like Keratinocytes : "Insights into Similarity with Psoriatic Lesional Epidermis" / Ghaffarinia Ameneh, Póliska Szilárd, Ayaydin Ferhan, Goblos Aniko, Parvaneh Shahram, Manczinger Máté, Balogh Fanni, Erdei Lilla, Veréb Zoltán, Szabó Kornélia, Bata-Csörgő Zsuzsanna, Kemény Lajos
Dátum:	2023
ISSN:	2073-4409
Megjegyzések:	Abstract: Keratinocytes are one of the primary cells affected by psoriasis inflammation. Our study aimed to delve deeper into their morphology, transcriptome, and epigenome changes in response to psoriasis-like inflammation. We created a novel cytokine mixture to mimic mild and severe psoriasis-like inflammatory conditions in cultured keratinocytes. Upon induction of inflammation, we observed that the keratinocytes exhibited a mesenchymal-like phenotype, further confirmed by increased VIM mRNA expression and results obtained from confocal microscopy. We performed RNA sequencing to achieve a more global view, revealing 858 and 6987 DEGs in mildly and severely inflamed keratinocytes, respectively. Surprisingly, we found that the transcriptome of mildly inflamed keratinocytes more closely mimicked that of the psoriatic epidermis transcriptome than the severely inflamed keratinocytes. Genes involved in the IL-17 pathway were a major contributor to the similarities of the transcriptomes between mildly inflamed KCs and psoriatic epidermis. Mild and severe inflammation led to the gene regulation of epigenetic modifiers such as HATs, HDACs, DNMTs, and TETs. Immunofluorescence staining revealed distinct 5-hmC patterns in inflamed versus control keratinocytes, and consistently low 5-mC intensity in both groups. However, the global DNA methylation assay detected a tendency of decreased 5-mC levels in inflamed keratinocytes versus controls. This study emphasizes how inflammation severity affects the transcriptomic similarity of keratinocytes to psoriatic epidermis and proves dynamic epigenetic regulation and adaptive morphological changes in inflamed keratinocytes.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk psoriasis keratinocyte cytokine inflammation transcriptome
Megjelenés:	Cells. - 12 : 24 (2023), p. 1-24. -
További szerzők:	Póliska Szilárd (1978-) (biológus) Ayaydin, Ferhan Goblos Anikó Parvaneh, Shahram Manczinger Máté Balogh Fanny (1995-) (hallgató) Erdei Lilla Veréb Zoltán (1980-) (immunológus, mikrobiológus, molekuláris biológus) Szabó Kornélia Bata-Csörgő Zsuzsanna Kemény Lajos V. (bőrgyógyász Szeged)
Pályázati támogatás:	TKP2021-NKTA-34 FIKP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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