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001-es BibID:	BIBFORM120798
035-os BibID:	(Scopus)85192715341 (WoS)001219985100001
Első szerző:	Horváth Ádám
Cím:	Anti-Nociceptive Effects of Sphingomyelinase and Methyl-Beta-Cyclodextrin in the Icilin-Induced Mouse Pain Model / Ádám Horváth, Anita Steib, Andrea Nehr-Majoros, Boglárka Kántás, Ágnes Király, Márk Racskó, István Balázs Tóth, Eszter Szánti-Pintér, Eva Kudová, Rita Skoda-Földes, Zsuzsanna Helyes, Éva Szőke
Dátum:	2024
ISSN:	1422-0067
Megjegyzések:	The thermo- and pain-sensitive Transient Receptor Potential Melastatin 3 and 8 (TRPM3 and TRPM8) ion channels are functionally associated in the lipid rafts of the plasma membrane. We have already described that cholesterol and sphingomyelin depletion, or inhibition of sphingolipid biosynthesis decreased the TRPM8 but not the TRPM3 channel opening on cultured sensory neurons. We aimed to test the effects of lipid raft disruptors on channel activation on TRPM3- and TRPM8-expressing HEK293T cells in vitro, as well as their potential analgesic actions in TRPM3 and TRPM8 channel activation involving acute pain models in mice. CHO cell viability was examined after lipid raft disruptor treatments and their effects on channel activation on channel expressing HEK293T cells by measurement of cytoplasmic Ca2+ concentration were monitored. The effects of treatments were investigated in Pregnenolone-Sulphate-CIM-0216-evoked and icilin-induced acute nocifensive pain models in mice. Cholesterol depletion decreased CHO cell viability. Sphingomyelinase and methyl-beta-cyclodextrin reduced the duration of icilin-evoked nocifensive behavior, while lipid raft disruptors did not inhibit the activity of recombinant TRPM3 and TRPM8. We conclude that depletion of sphingomyelin or cholesterol from rafts can modulate the function of native TRPM8 receptors. Furthermore, sphingolipid cleavage provided superiority over cholesterol depletion, and this method can open novel possibilities in the management of different pain conditions.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk cholesterol lipid raft methyl-beta-cyclodextrin pain sphingomyelinase Transient Receptor Potential
Megjelenés:	International Journal Of Molecular Sciences. - 25 : 9 (2024), p. 4637. -
További szerzők:	Steib Anita Nehr-Majoros Andrea Kántás Boglárka Király Ágnes Racskó Márk (1991-) (molekuláris biológus) Tóth István Balázs (1978-) (élettanász) Szánti-Pintér Eszter Kudová, Eva Skoda-Földes Rita Helyes Zsuzsanna Szőke Éva
Pályázati támogatás:	FK 134725 OTKA GINOP-2.3.2-15-2016-00050 GINOP TKP2021-EGA-13 Egyéb TKP2021-EGA-16 Egyéb ÚNKP-22-3-I-DE-324 Egyéb ÚNKP-23-3-II-DE-430 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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