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001-es BibID:	BIBFORM120976
035-os BibID:	(Scopus)85194247733 (WoS)001233655800001
Első szerző:	Szűcs Zsolt (gyógyszerész)
Cím:	Production of Polyphenolic Natural Products by Bract-Derived Tissue Cultures of Three Medicinal Tilia spp.: A Comparative Untargeted Metabolomics Study / Zsolt Szűcs, Zoltán Cziáky, László Volánszki, Csaba Máthé, Gábor Vasas, Sándor Gonda
Dátum:	2024
ISSN:	2223-7747
Megjegyzések:	Medicinal plant tissue cultures are potential sources of bioactive compounds. In this study, we report the chemical characterization of the callus cultures of three medicinal Tilia spp. (Tilia cordata, Tilia vulgaris and Tilia tomentosa), along with the comparison to bracts and flowers of the same species. Our aim was to show that calli of Tilia spp. are good alternatives to the calli of T. americana for the production of polyphenols and are better sources of a subset of polyphenolic metabolites, compared to the original organs. Calli were initiated from young bracts and grown on woody plant medium containing 1 mg L?1 2,4-D and 0.1 mg L?1 BAP. For chemical characterization, a quality-controlled untargeted metabolomics approach and the quantification of several bioactive compounds was performed with the use of LC-ESI-MS/MS. While bracts and flowers contained flavonoid glycosides (astragalin, isoquercitrin) as major polyphenols, calli of all species contained catechins, coumarins (fraxin, esculin and scopoletin) and flavane aglyca. T. tomentosa calli contained 5397 ?g g DW?1 catechin, 201 ?g g DW?1 esculin, 218 ?g g DW?1 taxifolin and 273 ?g g DW?1 eriodictyol, while calli from other species contained lower amounts. T. cordata and T. tomentosa flowers were rich in isoquercitrin, containing 8134 and 6385 ?g g DW?1, respectively. The currently tested species contained many of the bioactive metabolites described from T. americana. The production of catechin was shown to be comparable to the most efficient tissue cultures reported. Flowers and bracts contained flavonoid glycosides, including tiliroside, resembling bioactive fractions of T. americana. In addition, untargeted metabolomics has shown fingerprint-like differences among species, highlighting possible chemotaxonomic and quality control applications, especially for bracts.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk linden lime tree plant tissue culture natural products in vitro culture
Megjelenés:	Plants-Basel. - 13 : 10 (2024), p. 1-19. -
További szerzők:	Cziáky Zoltán Volánszki László (1994-) (Gyógyszerész) Máthé Csaba (1966-) (biológus) Vasas Gábor (1975-) (biológus-vegyész) Gonda Sándor (1984-) (gyógyszerész)
Pályázati támogatás:	142146 OTKA 146030 OTKA Bolyai János Ösztöndíj Egyéb ÚNKP-23-5 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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