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001-es BibID:	BIBFORM122876
035-os BibID:	(WoS)001256741000001 (Scopus)85197878134
Első szerző:	Kerek Ádám (állatorvos)
Cím:	Monitoring Changes in the Antimicrobial-Resistance Gene Set (ARG) of Raw Milk and Dairy Products in a Cattle Farm, from Production to Consumption / Ádám Kerek, Virág Németh, Ábel Szabó, Márton Papp, Krisztián Bányai, Gábor Kardos, Eszter Kaszab, Krisztina Bali, Zoltán Nagy, Miklós Süth, Ákos Jerzsele
Dátum:	2024
ISSN:	2306-7381
Megjegyzések:	Raw milk and dairy products can serve as potential vectors for transmissible bacterial, viral and protozoal diseases, alongside harboring antimicrobial-resistance genes. This study monitors the changes in the antimicrobial-resistance gene pool in raw milk and cheese, from farm to consumer, utilizing next-generation sequencing. Five parallel sampling runs were conducted to assess the resistance gene pool, as well as phage or plasmid carriage and potential mobility. In terms of taxonomic composition, in raw milk the Firmicutes phylum made up 41%, while the Proteobacteria phylum accounted for 58%. In fresh cheese, this ratio shifted to 93% Firmicutes and 7% Proteobacteria. In matured cheese, the composition was 79% Firmicutes and 21% Proteobacteria. In total, 112 antimicrobial-resistance genes were identified. While a notable reduction in the resistance gene pool was observed in the freshly made raw cheese compared to the raw milk samples, a significant growth in the resistance gene pool occurred after one month of maturation, surpassing the initial gene frequency. Notably, the presence of extended-spectrum beta-lactamase (ESBL) genes, such as OXA-662 (100% coverage, 99.3% identity) and OXA-309 (97.1% coverage, 96.2% identity), raised concerns; these genes have a major public health relevance. In total, nineteen such genes belonging to nine gene families (ACT, CMY, EC, ORN, OXA, OXY, PLA, RAHN, TER) have been identified. The largest number of resistance genes were identified against fluoroquinolone drugs, which determined efflux pumps predominantly. Our findings underscore the importance of monitoring gene pool variations throughout the product pathway and the potential for horizontal gene transfer in raw products. We advocate the adoption of a new approach to food safety investigations, incorporating next-generation sequencing techniques.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk antimikrobiális rezisztencia tejtermék NGS antimicrobial-resistance genes raw milk dairy products next-generation sequencing cattle food safety public health ESBL taxonomy
Megjelenés:	Veterinary Sciences. - 11 : 6 (2024), p.1-16. -
További szerzők:	Németh Virág Szabó Ábel Papp Márton (bioinformatika) Bányai Krisztián (virológus) Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Kaszab Eszter (1989-) (biológus) Bali Krisztina Nagy Zoltán Süth Miklós Jerzsele Ákos
Pályázati támogatás:	RRF-2.3.1-21-2022-00001 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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