Találati lista | Tudóstér

001-es BibID:	BIBFORM123881
035-os BibID:	(WoS)001272951700001
Első szerző:	Drezek, Karolina
Cím:	Novel Saccharomyces uvarum x Saccharomyces kudriavzevii synthetic hybrid with enhanced 2-phenylethanol production / Karolina Drężek, Zsuzsa Antunovics, Agnieszka Karolina Grabiec
Dátum:	2024
ISSN:	1475-2859
Megjegyzések:	Background Over the last two decades, hybridization has been a powerful tool used to construct superior yeastfor brewing and winemaking. Novel hybrids were primarily constructed using at least one Saccharomyces cerevisiaeparent. However, little is known about hybrids used for other purposes, such as targeted flavor production, for exam-ple, 2-phenylethanol (2-PE). 2-PE, an aromatic compound widely utilised in the food, cosmetic, and pharmaceuticalindustries, presents challenges in biotechnological production due to its toxic nature. Consequently, to enhanceproductivity and tolerance to 2-PE, various strategies such as mutagenesis and genetic engineering are extensivelyexplored to improved yeast strains. While biotechnological efforts have predominantly focused on S. cerevisiae for 2-PEproduction, other Saccharomyces species and their hybrids remain insufficiently described.Results To address this gap, in this study, we analysed a new interspecies yeast hybrid, II/6, derived from S. uvarumand S. kudriavzevii parents, in terms of 2-PE bioconversion and resistance to its high concentration, comparing itwith the parental strains. Two known media for 2-PE biotransformation and three different temperatures were usedduring this study to determine optimal conditions. In 72 h batch cultures, the II/6 hybrid achieved a maximumof 2.36 ? 0.03 g/L 2-PE, which was 2?20 times higher than the productivity of the parental strains. Our interest laynot only in determining whether the hybrid improved in productivity but also in assessing whether its susceptibilityto high 2-PE titers was also mitigated. The results showed that the hybrid exhibited significantly greater resistanceto the toxic product than the original strains.Conclusions The conducted experiments have confirmed that hybridization is a promising method for modifyingyeast strains. As a result, both 2-PE production yield and tolerance to its inhibitory effects can be increased. Further-more, this strategy allows for the acquisition of non-GMO strains, alleviating concerns related to additional legislativerequirements or consumer acceptance issues for producers. The findings obtained have the potential to contributeto the development of practical solutions in the future. (PDF) Novel Saccharomyces uvarum x Saccharomyces kudriavzevii synthetic hybrid with enhanced 2-phenylethanol production.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Hybrids Saccharomyces 2-phenylethanol Ehrlich pathway
Megjelenés:	Microbial Cell Factories. - 23 : 1 (2024), p. 203-213. -
További szerzők:	Grabiec, Agnieszka Karolina Antunovics Zsuzsa (1978-) (molekuláris biológus, genetikus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: