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001-es BibID:	BIBFORM125463
Első szerző:	Papp Fruzsina Réka (klinikai laboratóriumi kutató)
Cím:	Characteristics of Hyaluronan Metabolism During Myofibroblast Differentiation in Orbital Fibroblasts / Papp Fruzsina R., Katko Monika, Csiki Robert, Galgoczi Erika, Molnar Zsanett, Erdei Annamaria, Bodor Miklos, Steiber Zita, Ujhelyi Bernadett, Nagy Endre V.
Dátum:	2024
ISSN:	1552-5783
Megjegyzések:	PURPOSE: To study the impact of myofibroblast differentiation (MD) on hyaluronan (HA) turnover in orbital fibroblasts (OFs) focusing on the expression of its key enzymes and their potential implications in the pathogenesis of thyroid eye disease (TED). METHODS: Primary cultures of OFs were established from tissue samples (TED OFs, n = 4; non-TED OFs, n = 5). MD was induced by TGF-?1 (5 ng/mL). Measurements were performed after 24- and 72-hour treatments. The proliferation rate was determined by 5-bromo-2 -deoxyuridine (BrdU) incorporation. HA level and size were measured using an aggrecan-based ELISA-like method and agarose gel electrophoresis, respectively. mRNA expressions of myofibroblast markers and enzymes with a role in HA metabolism were determined using real-time PCR. RESULTS: Upregulation of type I collagen alpha1 chain, alpha-smooth muscle actin, and fibronectin indicated that OFs underwent MD after stimulation by TGF-?. After 72 hours, proliferation of untreated cultures declined, but it remained higher in myofibroblasts. Pericellular HA content, but not HA in the supernatant of myofibroblasts, increased compared to untreated cells. TGF-? was a potent stimulator of hyaluronan synthase 1 (HAS1) expression. The expression of hyaluronidase-1 and cell migration?inducing protein (CEMIP) diminished following MD, whereas the expression of transmembrane protein 2, the regulator of HA catabolism through CEMIP, was elevated. The size distribution of HA shifted toward a high-molecular-weight form following treatment with TGF-?. CONCLUSIONS: OFs undergoing MD are characterized by decreased HA turnover as a consequence of the inhibition of hyaluronidases and HAS1 induction. Our results suggest that hyaluronidases could be potential targets in the treatment of TED.
Tárgyszavak:	Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk thyroid eye disease hyaluronan myofibroblast hyaluronidase CEMIP TMEM2
Megjelenés:	Investigative Ophthalmology & Visual Science. - 65 : 13 (2024), p. 1-12. -
További szerzők:	Katkó Mónika (1980-) (biológus) Csiki Róbert (1997-) (gyógyszerész) Galgóczi Erika (1986-) (biológus) Molnár Zsanett Erdei Annamária (1976-) (belgyógyász) Bodor Miklós (1969-) (belgyógyász, endokrinológus) Steiber Zita (1977-) (orvos, szemész) Ujhelyi Bernadett (1981-) (szemész) Nagy Endre V. (1957-) (belgyógyász, endokrinológus)
Pályázati támogatás:	143464 OTKA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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