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001-es BibID:	BIBFORM125833
Első szerző:	Timár József
Cím:	Functional genomics of calcium channels in human melanoma cells / Timár József, Deli Tamás, Ádám A., Csernoch László
Dátum:	2006
ISSN:	0960-8931
Megjegyzések:	Calcium signalling of human melanoma is in the focus of intensive research since the identification of the role of WNT signaling in melanogenesis. Genomic and functional studies pointed to the important role of various Ca2+ channels in melanoma, but these data were contradictory. Melanoma is one of the most aggressive human cancer which can colonize the host from a very small primary. On the other hand, melanoma is characterized by an apoptosis-resistant pheno-genotype resulting in resistance to chemotherapy. We have used DNA microarray technology to profile human melanoma compared to benign tumor (nevi) and metastatic variants to non-metastatic ones to reveal genetic background of its geno-phenotype. For this purpose we have used 3 genetically distinct human melanoma cell lines grown in vitro and in vivo. Data indicated that during progression of melanocytes toward melanoma several genes involved in calcium signaling were found to be upregulated including the ryanodin receptor-2 (RYR2), P2X7R and sorcin. During the metastatic progression, beside further upregulation of the P2X7R receptor another set of calcium-signaling genes were found to be upregulated such as T-type Ca-channel-1, sorting nexin and calneuron-1. Functional studies revealed that the RYR2 is not involved directly in Ca-signaling which is predominantly mediated by the purinergic P2X7R receptor. Activation of the RYR2 resulted in stimulation of cell proliferation, while activation of P2X7R by ATP inhibited apoptosis. On the other hand, P2X7R inhibition by Zn++ induced strong inhibition of cell proliferation of human melanoma cell lines partialy mediated by induction of apoptosis. These studies provide the first evidences for the crucial role for calcium signaling in the progression of human melanoma and may provide novel therapeutic targets.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idézhető absztrakt folyóiratcikk functional genomics calcium channels ryanodine receptor human melanoma cells
Megjelenés:	Melanoma Research. - 16 (2006), p. p S99-S100. -
További szerzők:	Deli Tamás (1979-) (szülész-nőgyógyász, endokrinológus szakorvos) Ádám A. Csernoch László (1961-) (élettanász)
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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