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001-es BibID:	BIBFORM126227
Első szerző:	Csaholczi Bianka
Cím:	Transglutaminase 2 is an RNA-binding protein : experimental verification and characterisation of a novel transglutaminase feature / Bianka Csaholczi, Anna Renáta Csuth, Ilma Rita Korponay-Szabó, László Fésüs, Róbert Király
Dátum:	2024
ISSN:	1742-464X 1742-4658
Megjegyzések:	Transglutaminase 2 (TG2) is a uniquely versatile protein with diverse catalytic activities, such as transglutaminase, protein disulfide isomerase, GTPase and protein kinase, and participates in several biological processes. According to information available in the RBP2GO database, TG2 can act as an RNA-binding protein (RBP). RBPs participate in posttranscriptional gene expression regulation, therefore influencing the function of RNA, while RNA molecules can also modulate the biological activity of RBPs. Our goal was to confirm this novel characteristic of TG2 in human umbilical cord vein endothelial cells (HUVEC), which physiologically express TG2. First, UV cross-linked RNA-protein complexes were isolated from immortalised HUVECs using orthogonal organic phase separation. Compared with the RBP2GO database, mass spectrometry identified 392 potential RBPs, including TG2 and 20 previously undescribed, endothelium-related RBPs. Recombinant human TG2 was also pulled down by magnetic bead immobilised total RNA from HUVEC. Complex formation between TG2 and a 43-mer RNA molecule with a secondary structure as well as a homo-oligomeric single-stranded poly(dG), but not poly(dA), could be observed in magnetic RNA-protein pull-down experiments. Experiments with TG2 inhibitors NC9 and GTP?S, which stabilise its open and closed conformation, respectively, revealed that the open conformation of the enzyme favoured RNA-binding. Biolayer interferometry revealed a high binding affinity between TG2 and RNA with a KD value of 88 nM. Based on modelling and site-directed mutagenesis studies, we propose that superficial residues on the catalytic core domain (173-177 amino acids), present in a hidden position in the closed TG2 conformation, are involved in RNA binding. Our study demonstrates TG2's previously uncharacterised RNA-binding ability, opening new avenues for understanding its multi-functionality.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk transglutaminase 2 RNA-binding RNA-binding protein endothelial cells
Megjelenés:	The FEBS Journal. - "Accepted by Publisher" (2024). -
További szerzők:	Csuth Anna Renáta Korponay-Szabó Ilma (1959-) (gyermekgyógyász) Fésüs László (1947-) (orvos biokémikus) Király Róbert (1975-) (biológus)
Pályázati támogatás:	NKFI OTKA K 129139 Egyéb
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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