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001-es BibID:BIBFORM004633
035-os BibID:(scopus)0344447088 (wos)000074651400008
Első szerző:Nagy Péter (biofizikus)
Cím:Intensity-based energy transfer measurements in digital imaging microscopy / Nagy P., Vamosi G., Bodnar A., Lockett S. J., Szollosi J.
Dátum:1998
ISSN:175-7571
Megjegyzések:Investigation of protein-protein associations is important in understanding structure and function relationships in living cells. Using Forster-type resonance energy transfer between donor and acceptor labeled monoclonal antibodies we can assess the cell surface topology of membrane proteins against which the antibodies were raised. In our current work we elaborated a quantitative image microscopic technique based on the measurement of fluorescence intensities to calculate the energy transfer efficiency on a pixel-by-pixel basis. We made use of the broad excitation and emission spectrum of cellular autofluorescence for background correction of images. In addition to the reference autofluorescence images (UV background) we recorded three fluorescent images (donor, acceptor and energy transfer signal) of donor-acceptor double labeled samples, and corrected for spectral spillage of the directly excited donor and acceptor fluorescence into the energy transfer image. After careful image registration we were able to calculate the energy transfer efficiency on a pixel-by -pixel basis. In this paper, we also present a critical comparison between results obtained with this method and other approaches (photobleaching and flow cytometric energy transfer measurements)
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Antibodies
Antibodies, Monoclonal
Biophysics
Cell Line
chemistry
Comparative Study
Energy Transfer
Fluorescence
Human
Hungary
Image Processing, Computer-Assisted
immunology
Membrane Proteins
methods
Microscopy
Microscopy, Fluorescence
Models, Theoretical
Proteins
Signal Processing,Computer-Assisted
Spectrometry, Fluorescence
statistics and numerical data
Support, Non-U.S.Gov't
Megjelenés:European Biophysics Journal. - 27 : 4 (1998), p. 377-389. -
További szerzők:Vámosi György (1967-) (biofizikus) Dóczy-Bodnár Andrea (1970-) (biofizikus) Lockett, Steven J. Szöllősi János (1953-) (biofizikus)
Internet cím:elektronikus változat
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