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001-es BibID:	BIBFORM004644
Első szerző:	Brock Roland
Cím:	Rapid characterization of green fluorescent protein fusion proteins on the molecular and cellular level by fluorescence correlation microscopy / Brock, R., Vamosi, G., Vereb, G., Jovin, T. M.
Dátum:	1999
Megjegyzések:	Fluorescence correlation microscopy (FCM) was applied to characterize fusion proteins of the green fluorescent protein (GFP) on the cellular as well as molecular level within seconds in an integrated instrument. FCM combines the inherent sensitivity and high spatial resolution of fluorescence correlation spectroscopy with fluorescence imaging and micropositioning, thereby providing a spectrum of molecular information in the cellular context. Signatures of characteristic parameters derived from the autocorrelation functions served to distinguish a GFP fusion protein of the epidermal growth factor receptor from GFP fluorescence in the endoplasmic reticulum and cytoplasm. Diffusion constants measured for free transiently expressed GFP reproduced values reported previously with other techniques. The accessible concentration range extends from millions to only a few thousand molecules per cell, with single molecule detectability in the femtoliter detection volume. The detailed molecular characterization offered by FCM is fully compatible with automation in sample identification and detection, offering new possibilities for highly integrated high-throughput screening
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban analysis Animal Automation chemistry Cho Cells Cytoplasm Diffusion Endoplasmic Reticulum Epidermal Growth Factor Fluorescence Hamsters instrumentation Luminescent Proteins methods Microscopy Microscopy, Fluorescence Proteins Receptor, Epidermal Growth Factor Recombinant Fusion Proteins Sensitivity and Specificity Support, Non-U.S.Gov't Transfection ultrastructure
Megjelenés:	Proceedings of the National Academy of Sciences of the United States of America. - 96 : 18 (1999), p. 10123-10128. -
További szerzők:	Vámosi György (1967-) (biofizikus) Vereb György (1965-) (biofizikus, orvos) Jovin, Thomas M.
Internet cím:	elektronikus változat elektronikus változat
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