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001-es BibID:	BIBFORM004654
035-os BibID:	(scopus)0344199417 (wos)000083351400015
Első szerző:	Varga Tamás (biológus)
Cím:	Single-strand breaks in agarose-embedded chromatin of nonapoptotic cells / Varga, T., Szilagyi, I., Szabo, G.
Dátum:	1999
Megjegyzések:	Loop-size chromatin fragmentation frequently observed upon apoptotic cell death is thought to be initiated by ss nicks. Here we show that the agarose-embedded, deproteinized chromatin of normal, non-apoptotic murine and human cells, as well as yeast protoplasts, falls apart to 50-300 kb ss fragments upon heat denaturation, as revealed by urea-TAE field-inversion agarose gel electrophoresis resolving ss and ds fragments alike. These data were in line with S1digestion experiments. The nicks (gaps) observed are best explained either by enzymatic cleavages occurring upon cell lysis instantaneously or by preexisting discontinuities becoming manifest upon heat denaturation. These discontinuities go unnoticed in the usual nondenaturaing circumstances but seem to be inevitably present in any DNA preparation. The loop-size ds DNA fragmentation in apoptosis may be based on these pre-existing or "ready-to-go" (upon cell lysis) ss discontinuities of the normal cellular chromatin
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Animals Apoptosis Aspergillus Nuclease S1 Biophysics Cell Line Cells chemistry Chromatin Dna DNA Damage DNA Fragmentation DNA,Single-Stranded Electrophoresis, Agar Gel Electrophoresis, Gel, Pulsed-Field Heat Human Humans Hungary Mice Nucleic Acid Denaturation Research Saccharomyces cerevisiae Sepharose Support ultrastructure Urea
Megjelenés:	Biochemical and Biophysical Research Communications. - 264 : 2 (1999), p. 388-394. -
További szerzők:	Szilágyi Ildikó (orvos) Szabó Gábor (1953-) (biofizikus)
Internet cím:	DOI elektronikus változat
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