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001-es BibID:	BIBFORM006016
Első szerző:	Szöllősi János (biofizikus)
Cím:	Fluorescence energy transfer measurements on cell surfaces : a critical comparison of steady-state fluorimetric and flow cytometric methods / János Szöllősi, Lajos Trón, Sándor Damjanovich, Stephen H. Helliwell, Donna Arndt-Jovin, Thomas M. Jovin
Dátum:	1984
Megjegyzések:	The energy transfer efficiency E was measured between fluorescein-conjugated concanavalin A (Con A) and rhodamine-conjugated Con A bound to homogeneous tissue culture cells, the HK22 murine lymphoma cell line. Results from a flow cytometric energy transfer method (FCET) and two different steady-state fluorimeter methods were compared. The data were found to be in close agreement after careful correction of the steady-state fluorimetric measurements for contributions from dissociating ligand. The biological variability of the individual cells with respect to E was calculated using an error propagation analysis and were found to be less than the variability in the absolute amount of ligand binding per cell. FCET has a number of advantages over the fluorimetric measurements using suspensions of cells: (1) relatively labile receptor-ligand complexes can be measured; (2) the analysis can be restricted to undamaged cells by gating the data collection on the light-scattering signals; (3) heterogeneous populations of cells with respect to donor and acceptor topology can be distinguished by the correlation of E with other cellular parameters derived from additional signals or combinations thereof; and (4) the dynamics of donor-acceptor redistribution on subpopulations can be measured.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk analysis Animal Cell Line Cell Membrane Concanavalin A Energy Transfer Flow Cytometry Fluorescence Lymphoma Mathematics methods Mice Mice,Inbred Strains Microscopy,Fluorescence pathology physiology physiopathology Support,Non-U.S.Gov't
Megjelenés:	Cytometry. - 5 : 2 (1984), p. 210-216. -
További szerzők:	Trón Lajos (1941-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Helliwell, Stephen H. Arndt-Jovin, Donna J. Jovin, Thomas M.
Internet cím:	elektronikus változat
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