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001-es BibID:	BIBFORM006413
Első szerző:	Kósa Karolina (népegészségügyi szakember)
Cím:	The H-ras oncogene interferes with retinoic acid signaling and metabolism in NIH3T3 cells / Kosa, K., Jones, C. S., De Luca, L. M.
Dátum:	1995
ISSN:	0008-5472 (Print)
Megjegyzések:	We have previously shown that retinoic acid (RA) fails to induce transglutaminase C in H-ras transformed NIH-3T3 cells. Therefore, we investigated the effect of the H-ras oncogene on the metabolism of RA and on the expression of the cellular RA-binding protein I mRNA. HPLC analysis of the media and cell extracts demonstrated that H-ras-transformed cells metabolize RA to a much lesser extent than control cells, resulting in a higher concentration of RA in H-ras cells. Although inactive in endogenous transglutaminase induction, H-ras cell-associated RA was shown to be biologically available to induce activation of a reporter construct containing a retinoid response element and in stimulating transglutaminase activity in nontransfected cells. Cellular RA-binding protein I mRNA, supposedly involved in RA storage, was significantly increased in the H-ras-transformed cells. These data demonstrate that, even though H-ras-transformed cells accumulate up to 20 fold the concentration of RA as NIH-3T3 cells, they fail to show transglutaminase induction, suggesting that H-ras interferes with signal transduction by RA.
Tárgyszavak:	Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban 3T3 Cells/drug effects/physiology Animals Base Sequence Chromatography, High Pressure Liquid Culture Media Gene Expression Regulation/drug effects Genes, Reporter Genes, ras/drug effects/ physiology Mice Molecular Sequence Data RNA, Messenger/genetics Receptors, Retinoic Acid/genetics Signal Transduction/drug effects/ physiology Transfection Tretinoin/ metabolism/ pharmacology Tritium
Megjelenés:	Cancer Research. - 55 : 21 (1995), p. 4850-4854. -
További szerzők:	Jones, C. S. De Luca, Luigi M.
Internet cím:	elektronikus változat elektronikus változat
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