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001-es BibID:	BIBFORM010513
Első szerző:	Damante, Chiara A.
Cím:	Metal Loading Capacity of A beta N-Terminus : a Combined Potentiometric and Spectroscopic Study of Zinc(II) Complexes with A beta(1-16), Its Short or Mutated Peptide Fragments and Its Polyethylene Glycol-ylated Analogue / Chiara A. Damante, Katalin Ősz, Zoltán Nagy, Giuseppe Pappalardo, Giulia Grasso, Giuseppe Impellizzeri, Enrico Rizzarelli, Imre Sóvágó
Dátum:	2009
ISSN:	0020-1669
Megjegyzések:	Aggregation of the amyloidp-peptide (A beta) into insoluble fibirils is a key pathological event in Alzheimer's Disease (AD). There is now compelling evidence that metal binding to A beta is involved in AD pathogenesis. The amino acid region 1-16 is widely considered as the metal binding domain of A beta. In this work, we used a combined potentiometric, NMR, and electrospray ionization mass spectrometry (ESI-MS),approach to study the zinc(II) binding to a new polyethylene glycol (PEG)-conjugated peptide fragment encompassing the 1-16 amino acid sequence of A beta (A beta(1-16)PEG). Our results demonstrate for the first time that the A beta(1-16) is able to coordinate up to three zinc ions, all the histidyl residues acting as independent anchor sites. The study was complemented by systematically investigating the zinc(II) complexes of a series of shorter peptide fragments related to the A beta(1-16) sequence, namely, A beta(1-4), A beta(1-6), AcA beta(1-6), AcA beta(8-16)Y10A. The comparison of the whole results allowed the identification of the zinc(II) preferred binding sites within the longer M(1-16) amino acid sequence. Unlike copper(II) that prefers the N-terminal amino group as the main binding site, the zinc(II) is preferentially placed in the 8-16 amino acidic region of A beta(1-16).
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Inorganic Chemistry. - 48 : 21 (2009), p. 10405-10415. -
További szerzők:	Ősz Katalin (1975-) (vegyész) Nagy Zoltán (1974-) (vegyész) Pappalardo, Giuseppe Grasso, Giulia Impellizzeri, Giuseppe Rizzarelli, Enrico Sóvágó Imre (1946-) (vegyész)
Pályázati támogatás:	NKTH-OTKA K 68482 OTKA 77586 OTKA
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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