Találati lista | Tudóstér

001-es BibID:	BIBFORM011503
035-os BibID:	(cikkazonosító)208
Első szerző:	Balogh A.
Cím:	PPAR delta is the dominant PPAR isoform in UV exposed in vitro differentiated primary human keratinocytes / Balogh A., Törőcsik D., Nagy L., Remenyik É.
Dátum:	2009
ISSN:	0022-202X 1523-1747
Megjegyzések:	Nuclear receptors are transcription factors that bind directly to DNA. Upon activation by their lipid soluble ligands they alter the expression of their target genes, playing important roles in metabolic processes, proliferation, differentiation, apoptosis, and immune responses of the cells on the level of gene expression regulation. Our aim was to examine the role of nuclear receptors that are known to be expressed in the skin such as Peroxisome Proliferator- Activated Receptors (PPARs), Retinoic Acid Receptors (RARs), Retinoid X Receptors (RXRs) and Liver X Receptors (LXRs) in in vitro differentiated and in UV exposed keratinocytes.In the experiments we used primary keratinocytes differentiated by raising the Ca2+ levels of the culturing medium to 1.2mM in a 0-6 days period and exposed to UVB (30mJ/cm2) on the last day of differentiation. The changes in the expression levels of the marker genes of differentiation (TGM1, INV, PI3) and in the expression levels of the nuclear receptors and their target genes were followed by Real Time Quantitative RT-PCR technique. After verifying the differentiation of the keratinocytes by detecting the induction of the marker genes we measured the mRNA expression levels of nuclear receptors. We found that mRNA levels of PPAR alpha, RAR beta/delta, RAR gamma, RAR alpha, RXR alpha RXR beta and LXR apha did not change markedly. Measuring the expression levels of the known target genes for the PPAR pathways (ADRP, CPT1, FABP4, ABCG1) it was found that while PPAR beta/delta and alpha could be activated, PPAR gamma was inactive when using specifi c, synthetic activators. Although in vitro UV exposure of the keratinocyte cultures resulted in decrease both in the expression levels of PPAR isotypes and in the expression levels of their target genes, the responsiveness of PPAR beta/delta to its activator was still at high levels. Our results further confi rm the role of PPAR beta/delta in keratinocyte homeostasis.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idézhető absztrakt folyóiratcikk human keratinocytes PPAR isoform UV exposed
Megjelenés:	The Journal of Investigative Dermatology. - 129 : Suppl. 2 (2009), p. S35. -
További szerzők:	Töröcsik Dániel (1979-) (bőrgyógyász) Nagy László (1966-) (molekuláris sejtbiológus, biokémikus) Remenyik Éva (1956-) (bőrgyógyász)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: