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001-es BibID:	BIBFORM023651
Első szerző:	Pecsenye Katalin (biológus)
Cím:	Enzymatic responses of Drosophila melanogaster to long- and short-term exposures to ethanol / K. Pecsenye, K. Bokor, L. P. Lefkovitch, B. E. Giles, A. Saura
Dátum:	1997
Megjegyzések:	The e?ects of environmental ethanol on larva- to-pupa survival and on the activities of four enzymes were investigated in three Drosophila melanogaster strains. The strains had different allelic combinations at the Odh and Aldox loci on their third chromosomes, but they all carried the AdhS-GpdhF allelic combination on the second chromosome. Replicates of each of the strains were exposed to three different ethanol treat- ments: (i) no ethanol in the medium (control); (ii) 5% ethanol for a single generation (short-term exposure); (iii) 5% ethanol for 20 generations (long-term exposure). In all experiments, the activities of four enzymes (ADH, ODH, GPDH and AOX) were measured in larvae, pu- pae and adults. The results showed that (i) the larval and adult metabolic responses to environmental ethanol were different; (ii) enzyme activity changes under short-term exposure differed from those measured under long-term exposure; (iii) the activities of the allozymes common to all strains (ADH-S and GPDH-F), differed depending on the genetic background. Changes in larva- to-pupa survival were seen when the larvae of control and exposed lines of the three strains were confronted with various concentrations of ethanol. In all three strains, the exposed lines had signi-cantly higher initial survival rate and ethanol tolerance than the control lines. Strain-speci?c di?erences were observed in the ethanol tolerance of both types of line.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Alcohol dehydrogenase Drosophila Ethanol exposure Larva-to-pupa survival Metabolic response
Megjelenés:	Molecular and general genetics. - 255 : 3 (1997), p. 258-268. -
További szerzők:	Bokor Katalin Lefkovitch, L. P. Giles, B. E. Saura, Anssi
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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