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001-es BibID:	BIBFORM023739
Első szerző:	Szegedi Andrea (bőrgyógyász)
Cím:	Significant correlation between the CD63 assay and the histamine release assay in chronic urticaria / A. Szegedi, B. Irinyi, M. Gál, J. Hunyadi, K. Dankó, E. Kiss, S. Sipka, G. Szegedi, E. Gyimesi
Dátum:	2006
Megjegyzések:	Background Antibodies directed to the a subunit of the high affinity IgE receptorand the IgE molecule are proposed to be of pathogenetic relevance in a group ofpatients with chronic urticaria (CU). The diagnosis of autoimmune chronic urticaria(ACU) is difficult; the autologous serum skin test (ASST) seems to be a usefulscreening test, but reliable, additional confirmatory methods are needed.Objectives To assess the diagnostic value of a modified serum-induced basophilactivation test, the CD63 expression assay, in the diagnosis of ACU by comparingthe results of the CD63 assay with the results of the histamine release (HR) test,the ASST and serum levels of soluble CD40 ligand (sCD40L).Methods Using basophils from an atopic (DA) and a nonatopic (DNA) donor theactivity of sera of 72 patients with CU were measured in HR assay by enzymelinkedimmunosorbent assay and in CD63 expression assay by flow cytometry.An ASST was carried out in all patients; in 30 of the 72 patients sCD40L wasdetected and correlations were derived between the different assays. Sera of 20normal controls and 26 patients with systemic autoimmune diseases were alsotested in the HR assay and in the CD63 expression assay.Results Histamine-releasing activity was detected in the sera of 51% (DA) and 32%(DNA) of CU patients and 57% (DA) and 28% (DNA) of sera upregulated CD63expression on the surface of basophils from the different donors. There was asignificant correlation between the HR and the CD63 assays carried out on bothdonors, but the ASST showed a strong correlation with the HR assay only for basophilsfrom the DA. The serum level of sCD40L was significantly higher inpatients with CU compared with controls, but the difference between the autoimmuneand the nonautoimmune groups was not significant.Conclusions The CD63 expression assay seems to be a reliable functional test in thediagnosis of ACU, particularly if highly sensitive donor basophils are used, butthe determination of the sCD40L serum level was not sufficient to differentiate
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban CD63 assay chronic urticaria flow cytometry
Megjelenés:	British Journal of Dermatology. - 155 : 1 (2006), p. 67-75. -
További szerzők:	Irinyi Beatrix (1972-) (bőrgyógyász, allergológus) Gál Mónika Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus) Dankó Katalin (1952-2021) (belgyógyász, allergológus és klinikai immunológus) Kiss Emese (1960-) (belgyógyász, immunológus) Sipka Sándor (1945-) (laboratóriumi szakorvos) Szegedi Gyula (1936-2013) (belgyógyász, immunológus) Gyimesi Edit (1957-) (klinikai biokémikus, vegyész)
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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