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001-es BibID:	BIBFORM024100
Első szerző:	Szabó Gábor (biofizikus)
Cím:	Independent variations of myc amplification, inducibility, maturation, and proliferation states in HL60 / Szabo G., Bunce C., Brown G., Klein G., Sumegi J.
Dátum:	1988
Megjegyzések:	We have investigated the possible relationship between c-myc gene activity and other variable traits in HL60. In a panel of variant lines, a good correlation was observed between myc gene copy number and the level of myc mRNA. There was no correlation between myc amplification or expression and the resistance of the lines to induction of terminal neutrophilic or monocytic differentiation. Therefore, myc mRNA level does not appear to determine the ability of the variant HL60 lines to respond to inducers of differentiation. Flow cytometric analyses of the expression of a differentiation antigen (AGF 4.36) revealed stable negative and positive subpopulations in growing HL60. myc amplification, expression, and inducibility were identical in these subpopulations, suggesting that variation of these traits in HL60 sublines and variants is not due to maturation state differences. myc gene copy number was also identical in transferrin receptor positive (proliferating) and negative (resting) populations. These data contradict the notion that myc amplification has been important in determining the in vitro biological properties of HL60
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Cell Culture Cell Division cell growth cell strain hl 60 controlled study Dimethyl Sulfoxide Dna Flow Cytometry Fluorescent Antibody Technique Gene Amplification Gene Expression Regulation gene induction Human human cell In Vitro Leukemia,Myelocytic,Acute Nucleic Acid Hybridization oncogene myc phorbol 13 acetate 12 myristate priority journal Proteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-myc Proto-Oncogenes RNA,Messenger Support, Non-U.S.Gov't Support, U.S.Gov't,P.H.S. Tumor Cells, Cultured
Megjelenés:	Experimental Cell Research. - 175 : 2 (1988), p. 334-343. -
További szerzők:	Bunce, Chris Brown, Geoffrey Klein, George Sümegi János
Internet cím:	elektronikus változat Intézményi repozitóriumban (DEA) tárolt változat
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