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001-es BibID:	BIBFORM031291
Első szerző:	Brubel Réka
Cím:	Presence of pituitary adenylate cyclase activating polypeptide and its type I receptor in the rat kidney / Brubel R., Horvath G., Reglodi D., Lubics A., Tamas A., Kiss P., Laszlo E., Nemeth J., Mark L., Szakaly P.
Dátum:	2011
Megjegyzések:	Pituitary adenylate cyclase activating polypeptide (PACAP), a multifunctional neuropeptide,has 2 active forms, PACAP38 and PACAP27. It is now well-established that PACAPhas several actions also in peripheral organs, including renoprotective effects. The peptideitself has not been previously identified in the rat kidney. The first aim of our study was toidentify PACAP in the rat kidney using mass spectrometry and radioimmunoassay (RIA).Receptor mRNA and binding studies revealed the existence of all 3 PACAP receptors(PAC1, VPAC1, and VPAC2) in the kidney, but their exact localization in histologicsections was not evident. Because most of the cytoprotective effects of PACAP relate to itsspecific PAC1 receptor, our second aim was to identify the cell types wherein the PAC1receptor is expressed in the rat kidney. Mass spectrometry revealed the presence ofPACAP38 in the kidney. RIA measurements showed both PACAP38- and PACAP27-likeimmunoreactivities in kidney homogenates, with PACAP38 being dominant. Immunohistochemistryrevealed PAC1 receptor-like immunoreactivity in kidney sections, mainlyexpressed in cortical tubular epithelial cells. These results showed PACAP to beendogenously present in the kidney. The tubular localization of the PAC1 receptorprovides the basis for the renal effects of the peptide under physiologic and pathologicconditions.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban PACAP38 PACAP27 rat kidney receptor
Megjelenés:	Transplantation Proceedings. - 43 : 4 (2011), p. 1297-1299. -
További szerzők:	Horváth Gábor (Pécs) Reglődi Dóra (Idegtudományok) Lubics Andrea (Pécs) Tamás Andrea (Idegtudomány) (Pécs) Kiss Péter (Pécs) László E. Németh József (1954-) (vegyész, analitikus) Mark L. Szakály Péter
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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