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001-es BibID:	BIBFORM035095
Első szerző:	Balkay László (biofizikus)
Cím:	A novel method for measuring intracellular pH : effect of neutron irradiation on pHi of transformed cells / László Balkay, Teréz Márián, Miklós Emri, Lajos Trón
Dátum:	1992
ISSN:	1011-1344
Megjegyzések:	A new flow cytometric method was developed for measuring the intracellular pH (pHi) of mammalian cells using a fluorescent pH indicator dye 2',7-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF). Emission intensities (or their ratios) measured from BCECF-loaded cells can be converted into absolute pHi values using appropriate calibration curves. By comparison of several possible measuring and data evaluation procedures a double-ratio method was suggested as the most advantageous protocol to yield reliable intracellular pH data. This method allows pHi to be determined on a cell-by-cell basis corrected for cell volume and change in geometry of input-output optics of the flow cytometer. Our method applies a standard calibration curve and does not necessitate its reconstruction for each new set of measurements. Cells of the OKT-4 and OKT-8 hybridoma lines were exposed to neutron irradiation of different doses. Irradiated cells underwent a biphasic alkalinization; an instantaneous effect detected within 1.5 h was found to be intensified over 24 h. For the interpretation of data we suggest that the increase in cytoplasmic pH following neutron treatment is evoked by two mechanisms.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal of Photochemistry and Photobiology B-Biology. - 16 : 3-4 (1992), p. 367-375. -
További szerzők:	Márián Teréz (1950-) (radiobiológus) Emri Miklós (1962-) (fizikus) Trón Lajos (1941-) (biofizikus)
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