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001-es BibID:	BIBFORM036586
Első szerző:	Kirchmaier, Carl Maximilian
Cím:	Einfluss von SIN 1 auf Wechselwirkungen von Endothelzellen und thrombozyten = Effect of SIN 1 on the interactions of endothelial cells and thrombocytes / C. M. Kirchmaier, I. Altorjay, O. Bellinger, H. K. Breddin
Dátum:	1991
Megjegyzések:	A new method has been developed to investigate the direct effect of endothelial cells on platelet aggregation in the presence of cultured confluent human EC monolayers. The inside of the disk shaped cuvettes are covered by human umbilical vein ECs. The cuvettes rotate in the light beam of a photometer. In these cuvettes the effect of different aggregation inducers was inhibited in a concentration-dependent manner, e. g. for collagen at 0.5 microgram/ml, ADP at 5 x 10(-7) M, epinephrine at 5 x 10(-7) M and thrombin at 0.05 U/ml final concentration. (Platelet count 5 x 10(5)/microliters). Higher concentrations of the inducers led to irreversible platelet aggregation and were used for testing of antiplatelet drugs. In this system we detected a synergism between the antiaggregatory effect of the EC monolayer and that of SIN 1 (Cassella, Frankfurt/Main) the main metabolite of Molsidomine. 10 micrograms/ml PRP of SIN 1 alone partially inhibited platelet aggregation induced by 1 microgram/ml collagen and 10(-6) M ADP respectively in uncovered aggregation cuvettes. In the presence of an EC monolayer complete inhibition of platelet aggregation was obtained at a 5-fold final concentration of both inducers. After pretreatment of ECs with 1 mmol ASA over 30 min. the antiaggregatory effect of SIN 1 decreased, but was more pronounced (50%) than in uncovered cuvettes (25%)
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban EC monolayers ADP 5 Collagen cytochemical enzyme külföldön készült közlemény
Megjelenés:	Zeitschrift für Kardiologie 80 : Suppl. 5. (1991), p. 13-16. -
További szerzők:	Altorjay István (1954-) (belgyógyász, gasztroenterológus, onkológus) Bellinger, Oswald Breddin, Hans Klaus
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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